Volume 12, Issue 2 (2022)                   JMBS 2022, 12(2): 1-19 | Back to browse issues page

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Salehi Bakhtiari A, Etemadifar Z, Borhani M S. Production optimization of canthaxanthin pigment from radioresistant Dietzia maris by response surface methodology and evaluation of its effect on cell culture. JMBS 2022; 12 (2) :1-19
URL: http://biot.modares.ac.ir/article-22-37092-en.html
1- University of Isfahan
2- University of Isfahan, Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Hezarjerib St., Isfahan, IRAN , zetemadifar@gmail.com
3- Biology Department, Faculty of Sciences, Gonbad Kavous University, Golestan, Iran
Abstract:   (1178 Views)
Background: Carotenoids are biological antioxidants and play important roles in protecting the body from diseases and aging. Canthaxanthin is one of the most widely used carotenoids in the industry and medicine. This study aimed to investigate the biological properties of canthaxanthin pigment as well as its production optimization in a low-cost medium using a radioresistant microbial strain named Dietzia maris.
Materials and methods: Bacterial carotenoids were extracted and its antibacterial, anti-tumor, and cytotoxicity properties were investigated. Then, the effect of Krebs intermediates and pH on the production of pigment and microbial biomass in the whey medium was investigated using the response surface methodology.
Results: Maximum pigment production was found to be 92/54 mg/l in whey culture medium at pH 8 and in the presence of 12.5 mM of each of citrate, glutamate, malate, and succinate by the response surface method. The pigment did not show any cytotoxic effect on Hela, HFB, and MCF-7 cell lines. Besides, the pigment did not have any antibacterial properties.
Conclusion: Radioresistant microbial strains are better candidates for microbial pigment production due to their stability and high antioxidant activity. In this study, a whey culture medium was used to reduce the production cost of canthaxanthin. The addition of Krebs intermediaries in the fermentation medium increased the pigment production by Dietzia maris significantly.
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Article Type: Original Research | Subject: Microbial biotechnology
Received: 2019/10/6 | Accepted: 2020/12/30 | Published: 2022/01/30

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