Volume 11, Issue 4 (2020)
JMBS 2020, 11(4): 121-132 |
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Sheykhi E, Behnaz Shojaedin-Givi B, Tavaddod S, Bassam M A, Naderi-Manesh H, sajad B. Quantification of Superficial Distribution of Fluorophores in a Total-Internal-Reflection Fluorescence Microscope. JMBS 2020; 11 (4) :121-132
URL: http://biot.modares.ac.ir/article-22-38469-en.html
URL: http://biot.modares.ac.ir/article-22-38469-en.html
Elham Sheykhi1 
, Behnaz Behnaz Shojaedin-Givi2 , Sharareh Tavaddod3 , Mohammad amin Bassam4 , H. Naderi-Manesh5 , Batool Sajad * 6
, Behnaz Behnaz Shojaedin-Givi2 , Sharareh Tavaddod3 , Mohammad amin Bassam4 , H. Naderi-Manesh5 , Batool Sajad * 6
1- alzahra university PhD student
2- tarbiat modares university PhD student
3- edinburg university postdoc student, James Clerk Maxwell Building
4- malek ashtar university faculty
5- tarbiat modares unuversity faculty
6- alzahra university faculty ,bsajad@alzahra.ac.ir
2- tarbiat modares university PhD student
3- edinburg university postdoc student, James Clerk Maxwell Building
4- malek ashtar university faculty
5- tarbiat modares unuversity faculty
6- alzahra university faculty ,
Abstract: (1827 Views)
Total-Internal-Reflection Fluorescence Microscopy (TIRFM) is a useful tool to visualize and record the phenomena that happens below 100 nm thickness of the sample surface. This unique property of TIRFM help to perform a "qualitative" study of cytoskeleton near the cell-substrate contact. Here, distribution of actin filaments at cell-substrate interface was imaged by a TIRFM set up. Then, staining the actins cytoskeleton of the human melanoma cell and implementing the prism-based total-internal-reflection fluorescence microscope. A method to "quantify" distribution of fluorophores at cell-substrate contact is proposed.
Keywords: total-internal-reflection fluorescence microscopy, quantification, fluorophore, probability-distribution-function, surface-volume distribution
Article Type: Original Research |
Subject:
Animal biotechnology
Received: 2019/11/25 | Accepted: 2020/10/27 | Published: 2020/11/30
Received: 2019/11/25 | Accepted: 2020/10/27 | Published: 2020/11/30
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