Volume 13, Issue 1 (2022)                   JMBS 2022, 13(1): 47-64 | Back to browse issues page

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Chokan E, Vahidi B, khani M M. Computational analysis of micropipette aspiration technique on a stem cell. JMBS 2022; 13 (1) :47-64
URL: http://biot.modares.ac.ir/article-22-50487-en.html
1- University of Tehran
2- Associate professor/University of Tehran , bahman.vahidi@ut.ac.ir
3- Department of Tissue engineering and applied cell sciences, School of Advanced Technologies in Medicine, Shahid BeheshtiUniversity of Medical sciences,
Abstract:   (1154 Views)
Mechanical properties of living cells play an important role in helping to understand cell physiology and pathology. Evaluation of mechanical properties of cells may potentially lead to new mechanical diagnostic methods for some of these diseases. In this study, viscoelastic properties of the outer layer (cytoplasm and membrane) were extracted using standard linear solid model. Finite element modeling of the two cell layers is performed and the model is validated by experimental data. In the two-layer model, the effect of the radius of the nucleus and the location of the nucleus in the cell are investigated on the cell properties. By reducing the cytoplasmic radius ratio up to 43%, the whole cell properties follow the cytoplasmic properties and the effect of the nucleus can be neglected. The 50-second displacement change at a radial ratio of 0.53 increased to 4.5% compared to radial ratio of 1.58.  At a radial ratio of 0.43, a change in cell behavior was observed compared to the previous one, with a displacement change equals to 6.8% compared to radial ratio of 1.85 and a displacement reduction of 9.5% at a radial ratio of 0.53. The results demonstrate that the location of the nucleus and the ratio of the radius of the cytoplasm to the radius of the nucleus can effectively influence the viscoelastic properties and mechanical behavior of the cell.
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Article Type: Original Research | Subject: Biological system
Received: 2021/02/26 | Accepted: 2021/09/26 | Published: 2023/01/4

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