Volume 13, Issue 2 (2023)
JMBS 2023, 13(2): 80-94 |
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Ebrahimzadeh Kouchesfahani M, Bahrami A, babaeipour V. Production increasing of poly-γ-glutamic acid by batch and fed-batch cultures of Bacillus licheniformis ATCC 9945a and study of its physical and chemical structure. JMBS 2023; 13 (2) :80-94
URL: http://biot.modares.ac.ir/article-22-50974-en.html
URL: http://biot.modares.ac.ir/article-22-50974-en.html
1- Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Tehran, Iran
2- Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Tehran, Iran, Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Shabanlou Street, Lavizan, Shahid Babaei Highway, Tehran, Iran. P.O. Box: 15875-1774 ,a_bahrami@mut.ac.ir
3- Malek Ashtar University of Technology
2- Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Tehran, Iran, Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Shabanlou Street, Lavizan, Shahid Babaei Highway, Tehran, Iran. P.O. Box: 15875-1774 ,
3- Malek Ashtar University of Technology
Abstract: (1782 Views)
Poly-γ-glutamic acid (γ-PGA) is a beneficial, biocompatible, and biodegradable biopolymer. These properties have been led to the development of the use of this compound in various industries such as bio-medicine, biopharmaceutical, biotechnology, and tissue engineering. The limitation of the industrial development of γ-PGA is the high cost of its production. To reduce γ-PGA production costs, various strategies are used, such as culture medium optimization using inexpensive compounds, the development of efficient cultivation processes of batch and fed-batch. In this research, first, an efficient batch culture medium was developed to produce γ-PGA of Bacillus licheniformis ATCC 9945a. Then, the γ-PGA production increased by the pulsed feeding method and its optimization. By optimal culture medium development, the production of this product in batch culture was increased from 11 g/L to 47 g/L. Then, using the optimized pulsed feeding strategy of citrate (γ-PGA precursor), γ-PGA production was increased to 59.5 g/L, which is one of the highest production values reported with this strain. To optimize two-pulse feeding, the effect of feeding times, stock citrate solution concentration, and time of calcium and manganese solutions addition on γ-PGA production were investigated and optimized. Finally, FTIR confirmed the chemical structure of poly gamma glutamic acid, and the study of γ-PGA morphological properties with SEM showed a nanostructure ideal for biological applications.
Keywords: Submerged fermentation, Modified E culture medium, Pulse feeding strategy, Batch fermentation
Article Type: Original Research |
Subject:
Microbial biotechnology
Received: 2021/03/16 | Accepted: 2022/01/10 | Published: 2023/07/10
Received: 2021/03/16 | Accepted: 2022/01/10 | Published: 2023/07/10
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