Volume 13, Issue 1 (2022)                   JMBS 2022, 13(1): 121-136 | Back to browse issues page

‎ 20.1001.1.23222115.1400.13.1.8.7

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Mahdavian R, Naderi-Manesh H. A novel rearranged polydimethylsiloxane substrate for human fibroblast cells: The case of biomimetic effects on binding, morphology, and growth. JMBS 2022; 13 (1) :121-136
URL: http://biot.modares.ac.ir/article-22-55282-en.html
A novel rearranged polydimethylsiloxane substrate for human fibroblast cells: The case of biomimetic effects on binding, morphology, and growth
Reza Mahdavian1 , Hossein Naderi-Manesh * 2
1- Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
2- Professor, Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran,, Tarbiat Modares University, Jalal Al-e-Ahmad, Nasr, Tehran, Iran , naderman@modares.ac.ir
Abstract:   (2512 Views)
Cell substrates play a crucial role in tissue engineering and biomaterial science. Various studies are performed to develop the appropriate cell substrates for using in vitro and in vivo. Therefore, a biocompatible substrate that mimics the native extracellular matrix properties with specified surface topography as a biomimicry factor is necessary under ''the novel cell substrates development'' approaches. Our aim in the current study was to design, synthesize, and characterize a substrate with aligned nanometric arrays on the surface. The rapid and easy capabilities of Polydimethylsiloxane to receive chemical and physical characteristics with simple modifications, make it a promised candidate for the cell substrate. The obtained results from the atomic force and scanning electron microscopy showed the formation of 305±19 and 571±141 nanometers wrinkled nanoarrays after regulating the substrate under lateral traction during the plasma treatment times of 100 and 200s. Then, the behavior of a human foreskin fibroblast cell, in terms of adhesion, growth, viability, and morphology on this substrate was investigated. Increasing the plasma treatment time increased both nanoarray size and surface hydrophilicity, resulting in improved 17 and 46% of cell attachment quality, respectively. Additionally, the presence of the designed nanowrinkles surprisingly improved the number of the attached cells. The nanowrinkles caused the cells to align perfectly through the substrate's surface due to the contact guidance phenomena. Consequently, the biocompatible Polydimethylsiloxane substrate of this study with suitable chemical, mechanical, and physical properties showed fit capacities as a novel aligned cell culture platform. 
Keywords: Cell substrates, Cell morphology, Fibroblast cells, Nanowrinkles, Polydimethylsiloxane
Full-Text [PDF 1093 kb]   (1009 Downloads)    
Article Type: Original Research | Subject: Nanotechnology
Received: 2021/09/1 | Accepted: 2021/12/18 | Published: 2023/01/4
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