Volume 13, Issue 3 (2023)
JMBS 2023, 13(3): 83-92 |
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Gholami A, Zargar S J, tavakoli S. Anti-cancer effect of Oxypeucedanin methanolate purified from Ferulago trifida Boiss plant on A549. JMBS 2023; 13 (3) :83-92
URL: http://biot.modares.ac.ir/article-22-56820-en.html
URL: http://biot.modares.ac.ir/article-22-56820-en.html
1- Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran
2- Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran ,zargar@ut.ac.ir
3- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
2- Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran ,
3- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
Abstract: (670 Views)
Background: Lung adenocarcinoma is the most primary histologic subtype of non-small cell lung cancer (NSCLC). Oxypeucedanin methanolate, a member of furanocoumarin, is a naturally occurring compound, which is isolated from Ferulago trifida Boiss, an endemic species in North-West of Iran.
Purpose: We attempt to uncover the capacities of oxypeucedanin methanolate to induce apoptosis and autophagy in NSCLC cells, as well as the underlying mechanism involved in this process.
Methods: The effect of oxypeucedanin methanolate on cell viability was evaluated on A549 cells by MTT assay. Flow cytometry assay was used to detect cellular apoptosis. Expression levels of BAX, caspase-3, BCL2 and LC3 in A549 cells were measured by Real time quantitative reverse transcription-polymerase chain reaction (Real time RT-PCR). A549 cells migration were analyzed using a wound‐healing assay.
Results: Oxypeucedanin methanolate inhibited A549 cell proliferation in dose- and time- dependent manner, as evaluated by MTT assay. The total apoptosis rate was (5.46%) for A549 cells not treated with oxypeucedanin methanolate. In contrast, the apoptosis rate was (29.6%) for A549 cells treated with oxypeucedanin methanolate at the concentration of 0.4 mM. Real time RT-PCR revealed that the mRNA expression of BAX, caspase-3 and LC3 were upregulated, while mRNA expression of BCL2 was downregulated. Untreated cell migration increased significantly after 72 hours.
Conclusion: Oxypeucedanin methanolate inhibits proliferation and it could induce apoptosis and autophagy of human non-small cell lung cancer cell line A549. Oxypeucedanin methanolate may be a good candidate for reducing of A549 cells metastasis.
Purpose: We attempt to uncover the capacities of oxypeucedanin methanolate to induce apoptosis and autophagy in NSCLC cells, as well as the underlying mechanism involved in this process.
Methods: The effect of oxypeucedanin methanolate on cell viability was evaluated on A549 cells by MTT assay. Flow cytometry assay was used to detect cellular apoptosis. Expression levels of BAX, caspase-3, BCL2 and LC3 in A549 cells were measured by Real time quantitative reverse transcription-polymerase chain reaction (Real time RT-PCR). A549 cells migration were analyzed using a wound‐healing assay.
Results: Oxypeucedanin methanolate inhibited A549 cell proliferation in dose- and time- dependent manner, as evaluated by MTT assay. The total apoptosis rate was (5.46%) for A549 cells not treated with oxypeucedanin methanolate. In contrast, the apoptosis rate was (29.6%) for A549 cells treated with oxypeucedanin methanolate at the concentration of 0.4 mM. Real time RT-PCR revealed that the mRNA expression of BAX, caspase-3 and LC3 were upregulated, while mRNA expression of BCL2 was downregulated. Untreated cell migration increased significantly after 72 hours.
Conclusion: Oxypeucedanin methanolate inhibits proliferation and it could induce apoptosis and autophagy of human non-small cell lung cancer cell line A549. Oxypeucedanin methanolate may be a good candidate for reducing of A549 cells metastasis.
Article Type: Original Research |
Subject:
Biological system
Received: 2021/10/31 | Accepted: 2022/04/13 | Published: 2023/10/22
Received: 2021/10/31 | Accepted: 2022/04/13 | Published: 2023/10/22
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