1- tarbiat modares university
2- tarbiat modares university , rayeheh_vafaee@modares.ac.ir
Abstract: (7 Views)
PFU DNA polymerse shows the lowest error rate in Polymerase Chain Reaction (PCR) but it seperates from DNA after about 20 nucleotides add to the end of primer strand. The research purpus is processivity improvement of PFU DNA polymerase by means of rational design and point mutation due to lowest enthropy and enthalpy costs. so DNA polymerases in B family with high processivity were selected and their structures and sequences were compared with PFU DNA polymerase then an optimized mutation was induced . Native form and mutant were simulated for 100 ns and the trajectories were analyzed. ΔGbinding was calculated by g_mmpbsa tool and proved that the mutant shows a robust affinity .
Article Type:
Original Research |
Subject:
Bioinformatics Received: 2024/05/29 | Accepted: 2024/09/9