Study the biological production and antibacterial and antifungal effects of silver nanoparticles synthesized by the methanolic extract of clove (Syzygium aromaticum)

Study the biological production and antibacterial and antifungal effects of silver nanoparticles synthesized by the methanolic extract of clove (Syzygium aromaticum)

Abstract

Nanotechnology is a principally attractive area of research related with production of nanoparticles of variable sizes, shapes, chemical compositions and their possible application for human being benefits. Creation, manipulation and utilization of metallic nanoparticles, because of reduction of materials dimensions, affect the physical properties and results in displaying extraordinary thermal, optical and electronic properties of nonmaterial. The biological approaches to synthesis of nanoparticles are better than chemical and physical procedures because of low energy and time expenditure.

In this study the possibility of production of nano-silver particles from dried flower buds of Clove was investigated and antibacterial and anti-fungal activities of produced nanoparticles were studied by diffusion disc and well methods. The displayed UV-visible spectra, with a wavelength of 300 to 600 nm, identifies formation of silver nanoparticles, whenever the colorless initial acclimated mixture turned to brown. The centrifuged powder samples were examined using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) methods. Based on the results of this study, produced silver nanoparticles were spherical in the range of 27 to 69 nm and showed effective antimicrobial activity against Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Saccharomyces cerevisiae. Therefore clove can be used as a biological source for the synthesis of nanoparticles in an industrial scale with a very low cost.

Keywords

Antibacterial effect

anti-fungal effect

methanolic extract

clove

silver nanoparticles

Subjects

Agricultural Biotechnology

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