The Optimization of Culture Medium Bacillus thermoamylovorance Strain EAMYO by RSM and Improvement Biodesulfurization activity by Starch/Iron Nano Particles

Etemadi, Narges; Akhavan Sepahi, Abbas; Yazdian, Fatemeh; Mohebali, Ghasemali

The Optimization of Culture Medium Bacillus thermoamylovorance Strain EAMYO by RSM and Improvement Biodesulfurization activity by Starch/Iron Nano Particles

Document Type : Original Research

Authors

narges etemadi ¹

Abbas Akhavan sepahi ¹

fatemeh Yazdian ²

ghasemali mohebali ³

¹ Microbiology Group, Faculty of Life Science, North Tehran Branch, Islamic Azad University, Tehran, Iran.

² Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran

³ Microbiology and Biotechnology Research Group, Research Institute of Petroleum Industry, Tehran, Iran

Abstract

The combustion of fossil fuels containing sulfur results in the release of sulfur dioxide into the atmosphere and environmental pollution. Hence, the researchers focused on the biological desulfurization method. Dibenzothiophene is used as the model molecule to study the ability of the desulfurization of microorganisms. The most suitable sources of carbon, nitrogen and sulfur concentration optimized by response surface method to obtain the highest cell growth and biological desulfurization activity. The performance of iron nanoparticles on the growth and biodesulfurization activity of thermophilic bacterium Bacillus thermoamylovorans strain EAMYO was investigated. Characterization of starch-modified iron nanoparticles was performed by TEM, SEM. The images of TEM and SEM of starch / Iron nanoparticles showed that the Fe₃O₄ and Fe0 nanoparticles were 20 and 30 nm, respectively. The investigating the growth of microorganism in the presence of iron nanoparticles showed that these nanoparticles not only did not have a toxic effect on microorganism growth, but also increased the growth of microorganism in 96 h (OD 660 = 1.864, 1.896 respectively in the presence of nanoparticles Fe₀ and Fe₃O₄), while the highest rate of growth in the absence of nanoparticles in 96 h (OD660 = 1.51). Also, the activity of desulfurization in the presence of starch/Fe₀ nanoparticles and starch/Fe₃O₄ / starch increased by 26.52% and 10.75%, respectively, compared to the cells without the coating of iron nanoparticles.

Keywords

Thermophilic bacterium

Bio-desulfurization

RSM

Starch modified iron nanoparticles

20.1001.1.23222115.1399.11.2.3.4

Subjects

Industrial Biotechnology

[1] Mohebali, G., Ball, A.S., Rasekh, B., Kaytash, A. (2007). Biodesulfurization potential of a newly isolated bacterium, Gordonia alkanivorans RIPI90A. Enz. Microb. Technol. 40, 578–584.
[2] Kilbane, J.J., Bielaga, B.A. (1990) Toward sulfur-free fuels. Chem. Technol., 20, 747-751.
[3] Maxwell, S., Yu, J. (2000) Selective desulphurization of dibenzothiophene by a soil bacterium: microbial DBT desulphurization. Proc. Biochem. 35, 551–556.
[4] Kim, H.Y,. Kim, T.S,. Kim, H.B. (1990) Degredation of organic sulfur compounds and reduction of Dibenzothiophene to Biphenyl and hydrogen sulfide. Biotechnol. Lett. 2, 761-764.
[5] Kilbane, J.J., Jackowski, K. (1992) Biodesulfurization of water-soluble coal-derived material by Rhodococcus rodochrous strain IGTS8. Biotechnol. Bioeng. 40, 1107-1114.
[6] Kayser, K.J., Cleveland, L., Park, H., Kwak, J., Kolhatkar, A., Kilbane, J.J. (2002) Isolation and characterization of a moderate thermophile, Mycobacterium phlei GTIS10, capable of dibenzothiophene desulfurization. Appl. Microbiol. Biotechnol. 59, 737-746.
[7] Ansari, F., Grigoriev, P., Libor, S., Tothill, I.E., Ramsden, J.J. (2009) DBT degradation enhancement by decorating Rhodococcus erythropolis IGST8 with magnetic Fe3O4 nanoparticles. Biotechnol. Bioeng. 102 (5), 1505-1512.
[8] Shen, Y.F., Tang, J., Nie, Z.H. (2009) Preparation and application of magnetic Fe3O4 nanoparticles for wastewater purification. Sep. Pur. Technol. 68, 312–319.
[9] Guobin, S., JianMin, X., HuaiYing, Z., HuiZhou, L. (2005) Biodesulfurization of Dibenzothiophene by microbial cells coated with magnetite nanoparticles. Appl. Environ. Microbiol. 8, 4497-4502.
[10] Zhang, H.Y., Shan, G.B., Liu, H.Z., Xing, J.M. (2007) Surface modification of γ- Al2O3 nano- particles with gum Arabic and its applications in adsorption and biodesulfurization. Sur. Coat Technol. 201, 6917–6921.
[11] Etemadi, N., Akhavan Sepahy, A., Mohebali, G., Yazdian, F., Omidi, M. (2018) Enhancement of bio-desulfurization capability of a newly isolated thermophilic bacterium using starch/iron nanoparticles in a controlled system. Inter. J. Bio. Mac.120, 1801-1809.
[12] Bardania, H., Raheb, J., Mohammad-Beigi, H., Rasekh, B., Arpanaei, A. (2013) Desulfurization activity and reusability of magnetite nanoparticle-coated Rhodococcus erythropolis FMF and R. erythropolis IGTS8 bacterial cells. Biotechnol. Appl. Biochem. 60 (3), 323-329.
[13] Irani, Z.A., Mehrnia, M.R., Yazdian, F., Soheily, M., Mohebali, G., Rasekh, B. (2011) Analysis of petroleum biodesulfurization in an airlift bioreactor using response surface methodology. Bioresour. Technol. 102, 10585-10591.
[14] Karimi, E., C. Jeffryes, F. Yazdian, A. Akhavan Sepahi, A. Hatamian, B. Rasekh, H. Rashedi, M., Omidi, M.B. Ebrahim-Habibi, Ashrafi, J. (2017) DBT desulfurization by decorating Rhodococcus erythropolis IGTS8 using magnetic Fe3O4 nanoparticles in a bioreactor. Eng. Life Sci. 17, 528–535.
[15] Arabian, D., Najafi, H., Farhai, F., Dehkordi, A. (2014) Biodesulfurization of simulated light fuel oil by a native isolated bacteria Bacillus Cereus HN. J. Pet. Sci. Technol. 4(1), 31-40
[16] Jia X., Wen J., Sun Z., Caiyin Q., et al. (2006) Modeling of DBT Biodegradation Behaviors by Resting Cells of Gordonia sp. WQ- 01 and its Mutant in Oil-water Dispersions, Chem. Eng. Sci. 61,1987-2000.
[17] Hai, Y., Xudong, S., Qianqian, X., Zhao, M., Chengbin, X., Jun, N. (2008) Effects of nicotinamide and riboflavin on the biodesulfurization activity of dibenzothiophene by Rhodococcus erythropolis USTB-03. Environ. Sci. 20, 613-618.
[18] Derikvand, P., Etemadifar, Z., Saber, H. (2013). Optimization of nicotinamide and riboflavin in the biodesulfurization of dibenzothiophene using response surface methodology. Biol. J. Microo.1, 35-40.
[19] Mohebali, G., Ball, A.S. (2016). Biodesulfurization of diesel fuels e Past, present and future perspectives. Interna. Biodeterio. Biodegra. 110,163-180.
[20] Nigam, S., Barick, K.C., Bahadur, D. (2010). Development of citrate-stabilized Fe3O4 nanoparticles: Conjugationand release of doxorubicin for therapeutic applications. Elsevier B.V. All rights reserved.