Enhancement of native Aurantiochytrium sp.shy growth by medium optimization with response surface methodology

Pahlavan Yali, Morteza; Hjalili, Hjalili; Noroozi, Mostafa; Morady, Yazdan; Hallajisani, Ahmad

Enhancement of native Aurantiochytrium sp.shy growth by medium optimization with response surface methodology

Document Type : Original Research

Authors

Morteza Pahlavan Yali ¹

hjalili hjalili ²

Mostafa Noroozi ³

Yazdan Morady ⁴

Ahmad Hallajisani ⁵

¹ seafood processing at the Iranian Fisheries Sciences Research Institute

² Department of life science engineering, Faculty of new sciences and technologies, University of Tehran

³ Biotechnological Dep. faculty of Biological sciences, Alzahra University

⁴ Seafood Processing & Biotechnological Dep. Iranian Fisheries Sciences Research Institute

⁵ Caspian Faculty of Engineering, School of Engineering, University of Tehran

Abstract

The microalgal strain Aurantiochytrium sp. shy contains considerable amounts of poly-unsaturated fatty acids (PUFAs), chiefly docosahexaenoic acid (DHA) with potential pharmaceutical and health-related attributes. Effects of various concentrations of glucose, meat extract, monosodium glutamate and sea salt on the algal biomass and DHA production have been investigated in this study. Maximum algal biomass (7.1 g/l) was obtained when the culture medium contained 60 g/l of glucose, 6 g/l of meat extract, 6 g/l monosodium glutamate and sea salt at 25ppt. Lipid contents of the alga exceeded 30% of its dry cell weight, with palmitic acid and DHA as the most abundant components. When the effect of a single additive was concerned, meat extract was significantly effective, while interaction between meat extract and glucose was the most effective in comparison with other interactions (P < 0.0001). According to the results, glucose can assure more algal and fatty acids production when adequate amounts of meat extract exist in the culture medium. Optimal results attained when the ratios of glucose to meat extract and C/N concentrations were 10 and 5, respectively. Due to its remarkable growth rate and the capability to produce substantial quantities of biomass and fatty acids, Aurantiochytrium sp.shy was found to be a major source of the beneficial ingredients, whose productivity can magnify if its culture conditions is optimized using favorable blend of growth-promoting materials.

Keywords

Optimization

Biomass

response surface methodology

Microalgae Aurantiochytrium sp

20.1001.1.23222115.1399.11.4.2.7

Subjects

Industrial Biotechnology

Kim, S.-K., (2015), Springer handbook of marine biotechnology. Springer.
[2] Perez-Garcia, O. and Y. Bashan, (2015), Microalgal heterotrophic and mixotrophic culturing for bio-refining: from metabolic routes to techno-economics, in Algal biorefineries. Springer. 61-131.
[3] Chen, F., Y. Zhang, and S. Guo, (1996), Growth and phycocyanin formation of Spirulina platensis in photoheterotrophic culture. Biotechnology letters. 18(5): 603-608.
[4] Borowitzka, M.A., (1999) Commercial production of microalgae: ponds, tanks, and fermenters, in Progress in industrial microbiology. Elsevier. 313-321.
[5] Yokoyama, R. and D. Honda, (2007), Taxonomic rearrangement of the genus Schizochytrium sensu lato based on morphology, chemotaxonomic characteristics, and 18S rRNA gene phylogeny (Thraustochytriaceae, Labyrinthulomycetes): emendation for Schizochytrium and erection of Aurantiochytrium and Oblongichytrium gen. nov. Mycoscience, 48(4): 199.
[6] Fan, L. H., Zhang, Y. T., Cheng, L. H., Zhang, L., Tang, D. S. Chen, H. L. (2007), Optimization of carbon dioxide fixation by Chlorella vulgaris cultivated in a membrane‐photobioreactor. Chemical engineering & technology. 30(8): 1094-1099.
[7] Fekrat, F. and S. Shakeri, (2015), Investigation and production of omega 3 oil rich in docosahexaenoic acid by native strain of Aurantiochytrium TA4. Biological Journal of Microorganism. 4(14): 9-24.
[8] Standbury P.F., W., A.,Hall.S.J., (1986), Media For Industrial Fermentation - Priciples of Fermentation Technology. Pergamon -Oxford
[9] Ren, L.-J., LI, J., Hu, Y.-W., Ji, X.-J., Huang, H., (2013), Utilization of cane molasses for docosahexaenoic acid production by Schizochytrium sp. CCTCC M209059. Korean Journal of Chemical Engineering. 30(4): 787-789.
[10] Yamasaki, T., Aki, T., Shinozaki, M., Taguchi, M., Kawamoto, S., Ono, K., (2006), Utilization of Shochu distillery wastewater for production of polyunsaturated fatty acids and xanthophylls using thraustochytrid. Journal of bioscience and bioengineering. 102(4): 323-327.
[11] Chi, Z., Pyle, D., Wen, Z., Frear, C., Chen, S., (2007), A laboratory study of producing docosahexaenoic acid from biodiesel-waste glycerol by microalgal fermentation. Process Biochemistry, 42(11): 1537-1545.
[12] Aasen, I. M., Ertesvåg, H., Heggeset, T. M. B., Liu, B., Brautaset, T., Vadstein, O., Ellingsen, T. E., (2016), Thraustochytrids as production organisms for docosahexaenoic acid (DHA), squalene, and carotenoids. Applied microbiology and biotechnology. 100(10): 4309-4321.
[13] Sijtsma, L. and M. De Swaaf, (2004), Biotechnological production and applications of the ω-3 polyunsaturated fatty acid docosahexaenoic acid. Applied Microbiology and Biotechnology. 64(2): 146-153.
[14] Pahlavan yali, M., Jalili, H., Noroozi, M., Moradi, Y., Saba, F., (2017), optimization of culture condition for Growth of the Aurantiochytrium sp.shy Isolated from persian Gulf. Proceeding of 2thInternational and 10thnational Biotechnology congress of Islamic Repulic of Iran August 29-31 2017 Karaj-Iran, p. 78.
[15] Hong, D.D., H.T.L. Anh, and N.T.H. Thu, (2011), STUDY ON BIOLOGICAL CHARACTERISTICS OF HETEROTROPHIC MARINE MICROALGA—SCHIZOCHYTRIUM MANGROVEI PQ6 ISOLATED FROM PHU QUOC ISLAND, KIEN GIANG PROVINCE, VIETNAM1. Journal of Phycology. 47(4): 944-954.
[16] Yu, X.-J., Yu, Z.-Q., Liu, Y.-L., Sun, J., Zheng, J.-Y., Wang, Z., (2015), Utilization of high-fructose corn syrup for biomass production containing high levels of docosahexaenoic acid by a newly isolated Aurantiochytrium sp. YLH70. Applied Biochemistry and Biotechnology. 177(6): 1229-1240.
[17] Chaung, K.-C., Chu, C.-Y., Su, Y.-M., Chen, Y.-M., (2012), Effect of culture conditions on growth, lipid content, and fatty acid composition of Aurantiochytrium mangrovei strain BL10. AMB Express. 2(1): p. 42.
[18] Manikan, V., Kalil, M. S., Isa, M. H. M., Hamid, A. A., (2014), Improved prediction for medium optimization using factorial screening for docosahexaenoic acid production by Schizochytrium SP. SW1. American Journal of Applied Sciences. 11(3): 462-474.