Quantification of Superficial Distribution of Fluorophores in  a Total-Internal-Reflection Fluorescence Microscope

Sheykhi, Elham; Behnaz Shojaedin-Givi, Behnaz; Tavaddod, Sharareh; Bassam, Mohammad Amin; Naderi-Manesh, H.; Sajad, Batool

Quantification of Superficial Distribution of Fluorophores in a Total-Internal-Reflection Fluorescence Microscope

Document Type : Original Research

Authors

Elham Sheykhi ¹

Behnaz Behnaz Shojaedin-Givi ²

Sharareh Tavaddod ³

Mohammad amin Bassam ⁴

H. Naderi-Manesh ⁵

batool sajad ⁶

¹ alzahra university PhD student

² tarbiat modares university PhD student

³ edinburg university postdoc student

⁴ malek ashtar university faculty

⁵ tarbiat modares unuversity faculty

⁶ alzahra university faculty

Abstract

Total-Internal-Reflection Fluorescence Microscopy (TIRFM) is a useful tool to visualize and record the phenomena that happens below 100 nm thickness of the sample surface. This unique property of TIRFM help to perform a "qualitative" study of cytoskeleton near the cell-substrate contact. Here, distribution of actin filaments at cell-substrate interface was imaged by a TIRFM set up. Then, staining the actins cytoskeleton of the human melanoma cell and implementing the prism-based total-internal-reflection fluorescence microscope. A method to "quantify" distribution of fluorophores at cell-substrate contact is proposed.

Keywords

total-internal-reflection fluorescence microscopy

quantification

Fluorophore

probability-distribution-function

surface-volume distribution

20.1001.1.23222115.1399.11.4.6.1

Subjects

Animal biotechnology

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