The effect of graphitic carbon nitride on viability of Saos-2 cancer cell line

Ghasemi-Kasman, Maryam; Khakpour, Parisa; Ghasemi, Shahram; Pourbagher, Roghayeh

The effect of graphitic carbon nitride on viability of Saos-2 cancer cell line

Document Type : Original Research

Authors

Maryam Ghasemi-Kasman ¹

Parisa Khakpour ²

Shahram Ghasemi ³

Roghayeh Pourbagher ¹

¹ Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran

² Faculty of Pharmacy, Islamic Azad University, Ayatollah Amoli Branch, Amol, Iran

³ Faculty of Chemistry, University of Mazandaran, Babosarl, Iran

Abstract

Background and objective: In recent years, two-dimensional nanomaterials are widely used in biomedical applications. Graphitic carbon nitride has been significant not only due to good biocompatibility but also because of its application in the bio-imaging, diagnosis, and treatment of cancer. The purpose of this study was to investigate the effect of graphitic carbon nitride on viability of Saos-2 cancer cell line.

Methods: Graphitic carbon nitride was synthesized by adding melamine to hydrochloride acid and its physicochemical properties and structure was evaluated using FTIR, XRD, and Raman spectroscopy. Synthesized graphitic carbon nitride (50, 100, 200, 400, and 800 μg/mL) was added on Saos-2 cells and fibroblasts in two periods of 48 and 72 hours. The survival rate of the cells was investigated by MTT.

Findings: The results of FT-IR and XRD tests showed the structure and bonds of the synthesized compound, its accuracy and purity, respectively. Raman spectroscopy results also showed the graphitic content of the synthesized product. After passing 48 hours of the exposure of the cells to synthesized graphitic carbon nitride, the level of cell survival in the group that received 800 µg/mL was decreased to nearly 80% compared to the control (untreated) group.

Conclusion: The synthesized compound in this study might be considered as an appropriate option in cancer related researches.

Keywords

Graphitic carbon nitride

Cancer

Saos-2

Cell survival

20.1001.1.23222115.1400.12.3.10.0

Subjects

Nanotechnology

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