Anti-cancer effect of Oxypeucedanin methanolate purified from Ferulago trifida Boiss plant on A549

Gholami, Amene; Zargar, Seyed Jalal; Tavakoli, Saeed

Anti-cancer effect of Oxypeucedanin methanolate purified from Ferulago trifida Boiss plant on A549

Document Type : Original Research

Authors

Amene Gholami ¹

Seyed Jalal Zargar ¹

saeed tavakoli ²

¹ Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran

² Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran

Abstract

Background: Lung adenocarcinoma is the most primary histologic subtype of non-small cell lung cancer (NSCLC). Oxypeucedanin methanolate, a member of furanocoumarin, is a naturally occurring compound, which is isolated from Ferulago trifida Boiss, an endemic species in North-West of Iran.

Purpose: We attempt to uncover the capacities of oxypeucedanin methanolate to induce apoptosis and autophagy in NSCLC cells, as well as the underlying mechanism involved in this process.

Methods: The effect of oxypeucedanin methanolate on cell viability was evaluated on A549 cells by MTT assay. Flow cytometry assay was used to detect cellular apoptosis. Expression levels of BAX, caspase-3, BCL2 and LC3 in A549 cells were measured by Real time quantitative reverse transcription-polymerase chain reaction (Real time RT-PCR). A549 cells migration were analyzed using a wound‐healing assay.

Results: Oxypeucedanin methanolate inhibited A549 cell proliferation in dose- and time- dependent manner, as evaluated by MTT assay. The total apoptosis rate was (5.46%) for A549 cells not treated with oxypeucedanin methanolate. In contrast, the apoptosis rate was (29.6%) for A549 cells treated with oxypeucedanin methanolate at the concentration of 0.4 mM. Real time RT-PCR revealed that the mRNA expression of BAX, caspase-3 and LC3 were upregulated, while mRNA expression of BCL2 was downregulated. Untreated cell migration increased significantly after 72 hours.

Conclusion: Oxypeucedanin methanolate inhibits proliferation and it could induce apoptosis and autophagy of human non-small cell lung cancer cell line A549. Oxypeucedanin methanolate may be a good candidate for reducing of A549 cells metastasis.

Keywords

Non-Small Cell Lung Cancer

Coumarin

Oxypeucedanin methanolate

Apoptosis

Autophagy

20.1001.1.23222115.1401.13.3.8.5

Subjects

Biological system

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