Simple and rapid identification of aflatoxin B1 using a colorimetric aptasensor based on gold nanoparticles

Zeinoddini, Mehdi; Danesh, Abolfazl; Fadaee Kaghaki, Javad; Danesh, Normohammad

Simple and rapid identification of aflatoxin B1 using a colorimetric aptasensor based on gold nanoparticles

Document Type : Original Research

Authors

Mehdi Zeinoddini ¹

Abolfazl Danesh ²

Javad Fadaee kaghaki ³

Normohammad Danesh ¹

¹ Malek Ashtar University of Technology

² North Khorasan University of Medical Sciences

³ Faraja department of Law Enforcement Sciences

Abstract

Aflatoxin B1 is a type of mycotoxin produced by Aspergillus fungi during food production and storage. Aflatoxins have many toxic effects on the body that cause mutagens, teratogens and have high carcinogenic properties that cause cancer in the liver and other organs. Although conventional device methods for measuring aflatoxin B1 in food are sensitive and accurate, they have disadvantages such as high diagnostic time, high cost, the need for a trained user, and the creation of false positive results. Therefore, the development of new measuring methods has been prioritized by researchers. Among these measurement methods is the use of biosensors, which are fast, simple and more affordable and are used in the food industry today. In this work, a colorimetric optical aptasensor using gold nanoparticles with appropriate sensitivity and high selectivity was used to detect aflatoxin B1 in serum and buffer. For this purpose, gold nanoparticles were synthesized by reducing HAuCl₄ by sodium citrate (with a size of 14.40 nm and a zeta potential of -27.5). In this method, the protective effect of DNA sequence on the surface of gold nanoparticles has been used in the presence or absence of aflatoxin with the intervention of salt and the characteristic of visual color change. The detection limit of this method was estimated to be 50 ng/L and its linear range was 200-28000 ng/L. As a result, the designed aptasensor can be used for quick identification and screening of this toxin in contaminated food.

Keywords

Aflatoxin B1

Detection

Aptamer

Colorimetric

Gold Nanoparticles

Biosensor

20.1001.1.23222115.1401.13.4.11.0

Subjects

Nanotechnology

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