Design and fabrication of nanobioelectrochemical sensor based on metal-organic framework/graphene for breast cancer diagnosis

Zare, Ali-Akbar; Molaabasi, Fatemeh; Naderi-Manesh, Hossein

Design and fabrication of nanobioelectrochemical sensor based on metal-organic framework/graphene for breast cancer diagnosis

Document Type : Original Research

Authors

Ali-Akbar Zare ¹

Fatemeh Molaabasi ²

Hossein Naderi-Manesh ³

¹ Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

² Biomaterials and Tissue Engineering Research Group, Department of Interdisciplinary Technologies, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran

³ Department of Nanobiotechnology/Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

Abstract

Rapid and sensitive diagnosis of breast cancer, especially in the early stages of its formation, is very important. One of the methods of detecting cancer cells is the use of electrochemical sensors. Here, a new nanocomposite including an organic metal framework and silver nanoclusters are used. The resulting nanocomposite can be used as a scaffold to attach antibodies for the detection of HER2-positive cells. In the final nanocomposite structure, silver nanoclusters are placed in the internal cavities of the metal-organic framework, leading to strong electron transport, good biocompatibility, and high electrochemical activity. Our results showed that the designed electrochemical sensor has a high sensitivity in identifying HER2 positive cells, with a detection limit of 3 cells and a linear range of 100 to 5000 cells/ml. Also, the investigations showed that the introduced sensor has stability, good selectivity and acceptable application. The proposed strategy for the development of sensors based on metal-organic frameworks provides a promising approach for early detection of cancer markers and living cancer cells.

Keywords

Breast Cancer

Metal-organic framework

Ag nanocluster

Electrochemical sensor

Subjects

Nanotechnology

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