Differentially expressed piRNAs in breast cancer cells

Abedi, Maryam; Sadeghizadeh, Majid

Differentially expressed piRNAs in breast cancer cells

Document Type : Original Research

Authors

maryam abedi ¹

Majid Sadeghizadeh ²

¹ Ph.D. student, Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

² Professor, Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

Abstract

Breast cancer is the most common cancer in women, and despite many scientific advances, it remains the leading cause of cancer-related death in women. To solve this global problem, deeper molecular studies in the field of breast cancer are needed. Nowadays, the role of piRNAs in various cancers is of great interest. In this study, we aim to identify important piRNAs involved in breast cancer. For this purpose, raw small RNA seq data related to cancerous and normal breast tissue samples were selected and extracted from the GEO database, and the Galaxy platform was used for their bioinformatic analysis. The differential expression of 372 piRNAs was obtained based on Log2 FC ≥ 2, p-value ≤ 0.05, of which 191 showed increased expression and 181 showed decreased expression. The highest increase is related to hsa-piR-33125, whose target is GATAD2A and plays a role in carcinogenesis processes such as blood vessel development, apoptosis, regulation of gene expression at the transcriptional level, etc. The largest decrease is related to hsa-piR-33073 with Log2 FC= -4.20. To find a list of important piRNAs that have a significant difference in expression in breast cancer compared to normal tissue, as well as to determine the increase or decrease of their expression in cancer tissue and to identify the target genes and investigate their role in the biological pathways involved in the development and progression of cancer. This can be the beginning of studies that will ultimately lead to advances in breast cancer research and treatment methods.

Keywords

Breast Cancer

piRNA

differential expression

small RNA seq

molecular studies

Subjects

Bioinformatics

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