Lysinibacillus sp.: a suitable candidate for producing calcite and studying the efficiency of its bio-cementation potential

Ghezelbash, Gholam Reza; Karami, Arezoo

Lysinibacillus sp.: a suitable candidate for producing calcite and studying the efficiency of its bio-cementation potential

Document Type : Original Research

Authors

Gholam Reza Ghezelbash

Arezoo Karami

Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

The process of soil and concrete cementation through microbial induction depends on the efficiency of the enzymes in producing bacteria. The isolation of microorganisms with high urease activity may lead to the isolation of strains that are better at cementing in compression to the current strains. This research deals with the isolation of isolates with high urease ability. Isolate 233 out of 283 isolates was associated with the best urease activity, and its urease activity was the release of 5.04 micromoles of ammonium per millilitre per minute. This isolate using sequencing of a fragment of 16SrRNA and characterization of biochemical properties was identified as Lysinibacillus sp. strain 233 was deposited in NCBI under accession number OQ379213. The semi-quantitative XRD analysis of the bacterium's calcite crystals in the precipitation medium revealed that 87.1% were calcite while only 12.9% were vaterite. FESEM electron microscope images revealed cubic calcite crystals and EDX analysis confirmed the presence of its constituent elements, i.e., carbon, oxygen, and calcium.

Keywords

Calcite

Lysinibacillus sp

XRD

Urease activity

Subjects

Microbial biotechnology

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