Design and simulation of mRNA-9 interpreted in lung cancer using hydrogel platform

Tajik, Morvarid; Naderi-Manesh, Hossein; Allahverdi, Abdollah

Design and simulation of mRNA-9 interpreted in lung cancer using hydrogel platform

Authors

Morvarid Tajik ¹

Hossein Naderi-Manesh ²

Abdollah Allahverdi ³

¹ Biophysics Group, Faculty of Biological Science, Tehran, Iran

² Biophysics group, Faculty of Biological Sciences, Tarbiat Modares University, Tehran-Iran

³ Dept of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran- Iran

Abstract

Cancer is one of the causes of death in human societies, and the main reason for failure in its treatment is late diagnosis and involvement of other body organs. Biomarkers measurement of body fluids can be one of the most important methods of cancer screening and diagnosis in the early stages. Circulating microRNAs have been proposed as new biomarkers for cancer diagnosis and prognosis. The use of these molecules, in addition to early and timely diagnosis before metastasis, will reduce the damage to the patient due to the possibility of non-invasive access. Therefore, developing a method to identify, reveal and quantify it is a necessity. In this study, a biosensor was designed to isolate and identify circulating blood microRNAs in a hydrogel platform. In our work, microRNA was isolated using a single-stranded DNA receptor probe and fixed in a platform containing hydrogels. By attaching the microRNA to the probe, the second probe, which complements the biotinylated DNA at the top of the microRNA, forms a sandwich structure. Finally, microRNA trapped between the two probes was detected by FITC-bound streptavidin in the hydrogel platform. In this research, different concentrations of microRNA-9 from 1000 picomolar to 1 femtomol were used to estimate the limit of detection (LOD) of the designed biosensor, and 50 femtomol was measured as the detection limit.

Keywords

Biomarker

Chitosan

Streptavidin-FITC

Micro-RNA biosensor

Subjects

Molecular biotechnology

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