Investigations on various methods for cryopreservation of Echium amoenum Seeds

Ghaffarzadeh, Leila

doi:10.48311/biot.2025.103383.0

Investigations on various methods for cryopreservation of Echium amoenum Seeds

Document Type : Original Research

Author

Leila Ghaffarzadeh

university of mohaghegh ardabili

https://doi.org/10.48311/biot.2025.103383.0

Abstract

Echium amoenum is a member of the Boraginaceae family, which has very valuable medicinal properties that are highly regarded in traditional Iranian medicine. Because different plant species Echium inappropriate harvesting of natural areas by humans, and the growing threat of biotic and abiotic stresses following the work resulted in reduced genetic diversity of a species is, a species of genetically keep reserves Curb upper's. Using seed cryopreservation, as one of the Ex situ plant germplasm conservation method we can store seed for long-term, with much lower expenses and without losing seed viability. In order to preserve E. amoenum seed under -196 ºC condition, three pre cryopreservation treatments, PVS2 solution, Desiccation and Glycerol were applied. The treated seeds were transferred into Liquid Nitrogen (LN) and stored for 1 week. In this study the effect of cryopreservation on germination and growth indices (germination percent, germination rate of plant species (E. amoenum) in storage conditions of cryopreservation (-196ºC) were evaluated for 1 week. Comparing treatments, significant differences were observed on germination percent and germination rate. The best treatment was desiccation. The results showed that, the long-term preservation of the species, seed in -196 ºC is possible.

Keywords

Echium amoenum

Cryopreservation

Germplasm

Glycerol

PVS2

Subjects