1
Department of Biology, Ardabil Branch, Islamic Azad University, Ardabil, Iran
2
Department of Biology, Ard.C., Islamic Azad University, Ardabil, Iran
10.48311/biot.2026.118123.82882
Abstract
Targeted delivery of nucleic acids and drugs to diseased cells remains a major challenge in cancer therapy. This study focuses on Fe3O4/Chitosan/PCL/PEG-HA (PCFPH) nanoparticles designed to enhance PTX and siRNA delivery to HS-578T cells. Characterization by Fourier transform infrared spectroscopy (FTIR), zeta potential, dynamic light scattering (DLS), and scanning electron microscopy (SEM) confirmed the successful synthesis and structural properties suitable for drug and gene loading. The nanoparticles were measured to be approximately 230 nm with a zeta potential of -2.5 mV. Drug and gene release studies at pH=7.4 showed that the release of PTX and siRNA-FAM was controlled and biphasic. Electrophoretic analysis showed that the micellar coating protected these agents from plasma degradation. Cytotoxicity evaluation using MTT assay showed low toxicity (IC50= 492.7 μg/mL) of PCFPH nanocapsules for HS-578T cell line. Agarose gel and fluorescence microscopy results confirmed the superior gene delivery efficiency of PCFPH/siRNA-FAM nanocapsules compared to the control group, due to their stability and the presence of hyaluronic acid groups. This study presents the first application of these nanoparticles for gene and drug delivery.
yaghoubi,H. and Ajam,Z. (2025). Application of biocompatible copolymer nanoparticles in dual targeted delivery of siRNA and paclitaxel to HS-578T breast cancer cells. Modares Journal of Biotechnology, 17(1), 129-152. doi: 10.48311/biot.2026.118123.82882
MLA
yaghoubi,H. , and Ajam,Z. . "Application of biocompatible copolymer nanoparticles in dual targeted delivery of siRNA and paclitaxel to HS-578T breast cancer cells", Modares Journal of Biotechnology, 17, 1, 2025, 129-152. doi: 10.48311/biot.2026.118123.82882
HARVARD
yaghoubi H., Ajam Z. (2025). 'Application of biocompatible copolymer nanoparticles in dual targeted delivery of siRNA and paclitaxel to HS-578T breast cancer cells', Modares Journal of Biotechnology, 17(1), pp. 129-152. doi: 10.48311/biot.2026.118123.82882
CHICAGO
H. yaghoubi and Z. Ajam, "Application of biocompatible copolymer nanoparticles in dual targeted delivery of siRNA and paclitaxel to HS-578T breast cancer cells," Modares Journal of Biotechnology, 17 1 (2025): 129-152, doi: 10.48311/biot.2026.118123.82882
VANCOUVER
yaghoubi H., Ajam Z. Application of biocompatible copolymer nanoparticles in dual targeted delivery of siRNA and paclitaxel to HS-578T breast cancer cells. Modares Journal of Biotechnology, 2025; 17(1): 129-152. doi: 10.48311/biot.2026.118123.82882
