Volume 11, Issue 3 (2020)                   JMBS 2020, 11(3): 1-7 | Back to browse issues page

‎ 20.1001.1.23222115.1399.11.3.1.4

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Najmi Z, Ebrahimipour G. Biosutfactant production by an indigenous bacterium using molasses and partial characterization of produced biosurfactant. JMBS 2020; 11 (3) :1-7
URL: http://biot.modares.ac.ir/article-22-20038-en.html
Biosutfactant production by an indigenous bacterium using molasses and partial characterization of produced biosurfactant
Ziba Najmi * 1, Gholamhosein Ebrahimipour2
1- Department of Microbiology and Microbial Biotechnology, Faculty of Biological Science and Technology, Shahid Beheshti University , zibanajmi85@gmail.com
2- Department of Microbiology and Microbial Biotechnology, Faculty of Biological Science and Technology, Shahid Beheshti University
Abstract:   (4957 Views)
Although biosurfactants have great advantages over chemical surfactants, their wider industrial applications have been constrained by their relatively high production cost. Using renewable, sustainable and cheap substrates such as different industrial by-products and wastes maybe decrease biosurfactant production costs. Since in different countries, there are a variety of by-products and wastes so use of these substrates rely on their types and concentrations in countries. In addition to hydrocarbon compounds, molasses has been considered as a dominant by-product in Iran. In this study, among 16 crude oil degrading isolates, strain Pseudomonas aeruginosa ZN was selected as an efficient biosurfactant producer by screening methods for detection of biosurfactant producing bacteria. For investigation of molasses concentrations effect on bacterial growth and biosurfactant production, a wide range of molasses concentrations from 2-12% (v/v) were used. This strain was able to grow and produce biosurfactant in all range of molasses concentrations while the best concentrations were 4-6%. Also, at the optimum molasses concentration, reduction of surface tension from 70 to 32-34 mN/m was observed. The concnetrations more than these values decreased the growth and production process. Acid precipitation and solvent extract (ethyl acetate: hexane) methods were carried out for recovery of biosurfactant from the culture broth, then results of spraying on developed TLC and staining fermentation broth without bacterial cells showed the two produced biosurfactants were glycolipid.
Keywords: By-products, Molasses, Recovery, Partial detection.
Full-Text [PDF 480 kb]   (2744 Downloads)    
Article Type: Original Research | Subject: Agricultural Biotechnology
Received: 2017/01/31 | Accepted: 2017/08/14 | Published: 2020/06/9
References
1. [1] Pacwa-Płociniczak, M., Płaza, G. A., Piotrowska-Seget, Z., and Cameotra, S. S. (2011) Environmental applications of biosurfactants: recent advances, International Journal of Molecular Sciences 12, 633-654.
3. [2] Sekhon, K., Khanna, S., and Cameotra, S. (2011) Enhanced biosurfactant production through cloning of three genes and role of esterase in biosurfactant release, Microbial cell factories 10, 2-10.
5. [3] Tambekar, D., and Gadakh, P. (2013) BIOCHEMICAL AND MOLECULAR DETECTION OF BIOSURFACTANT PRODUCING BACTERIA FROM SOIL, life sciences biotechnology and pharma research 2, 204-211.
7. [4] Ebrahimipour, G., Gilavand, F., Karkhane, M., Kavyanifard, A., Teymouri, M., and Marzban, A. (2014) Bioemulsification activity assessment of an indigenous strain of halotolerant Planococcus and partial characterization of produced biosurfactants, International Journal of Environmental Science and Technology 11, 1379-1386.
9. [5] Belcher, R., Huynh, K., Hoang, T., and Crowley, D. (2012) Isolation of biosurfactant-producing bacteria from the Rancho La Brea Tar Pits, World Journal of Microbiology and Biotechnology 28, 3261-3267.
11. [6] Perfumo, A., Rudden, M., Smyth, T. J. P., Marchant, R., Stevenson, P. S., Parry, N. J., and Banat, I. M. (2013) Rhamnolipids are conserved biosurfactants molecules: implications for their biotechnological potential, Applied microbiology and biotechnology 97, 7297-7306.
13. [7] Franzetti, A., Bestetti, B., Caredda, P., La Colla, P., and Tamburini, E. (2008) Surface-active compounds and their role in the access to hydrocarbons in Gordonia strains, FEMS Microbiol Ecol 63, 238-248.
15. [8] Banat, I., Makkar, R., and Cameotra, S. (2000) Potential commercial applications of microbial surfactants, Applied microbiology and biotechnology 53, 495-508.
17. [9] Li, A. H., Xu, M. Y., Sun, W., and Sun, G. P. (2011) Rhamnolipid production by Pseudomonas aeruginosa GIM 32 using different substrates including molasses distillery wastewater, Applied biochemistry and biotechnology 163, 600-611.
19. [10] Mukherjee, S., Das, P., and Sen, R. (2006) Towards commercial production of microbial surfactants, Trends in Biotechnology 24, 509-515.
21. [11] Banat, I. M., Satpute, S. K., Cameotra, S. S., Patil, R., and Nyayanit, N. V. (2014) Cost effective technologies and renewable substrates for biosurfactants’ production, Frontiers in Microbiology 5.
23. [12] Maneerat, S. (2005) Production of biosurfactants using substrates from renewable-resources, Songklanakarin J. Sci. Technol 27, 675-683.
25. [13] Lazaridou, A., Roukas, T., Biliaderis, C. G., and Vaikousi, H. (2002) Characterization of pullulan produced from beet molasses by Aureobasidium pullulans in a stirred tank reactor under varying agitation, Enzyme and Microbial Technology 31, 122-132.
27. [14] Kalogiannis, S., Iakovidou, G., Liakopoulou-Kyriakides, M., Kyriakidis, D. A., and Skaracis, G. N. (2003) Optimization of xanthan gum production by Xanthomonas campestris grown in molasses, Process Biochemistry 39, 249-256.
29. [15] Skountzou, P., Soupioni, M., Bekatorou, A., Kanellaki, M., Koutinas, A. A., Marchant, R., and Banat, I. M. (2003) Lead(II) uptake during baker's yeast production by aerobic fermentation of molasses, Process Biochemistry 38, 1479-1482.
31. [16] Ikram-ul, H., Ali, S., Qadeer, M. A., and Iqbal, J. (2004) Citric acid production by selected mutants of Aspergillus niger from cane molasses, Bioresource Technology 93, 125-130.
33. [17] Shin, H. T., Baig, S. Y., Lee, S. W., Suh, D. S., Kwon, S. T., Lim, Y. B., and Lee, J. H. (2004) Production of fructo-oligosaccharides from molasses by Aureobasidium pullulans cells, Bioresource Technology 93, 59-62.
35. [18] Zulfiqar, A. R., Naseer, A., and Shahid, K. (2014) Multi-response optimization of rhamnolipid production using grey rational analysis in Taguchi method, Biotechnology Reports 3, 86-94.
37. [19] Carrillo, P., Mardaraz, C., Pitta-Alvarez, S., and Giuliett, A. (1996) Isolation and selection of biosurfactant producing bacteria, In Wortd Journal of Microbiology & Biotechnology, pp 82-84.
39. [20] Sneha, K., Padmapriya, B., and Rajeswari, T. (2012) Isolation and Screening of Biosurfactants Produced by Pseudomonas aeruginosa from Oil Spilled Soils, International Journal of Pharmaceutical & Biological 3, 321-325.
41. [21] Bodour, A., and Miller-Maier, R. (1998) Application of a modified drop-collapse technique for surfactant quantitation and screening of biosurfactant-producing microorganisms, Journal of Microbiological Methods 32, 273-280.
43. [22] Morikawa, M., Hirata, Y., and Imanaka, T. (2000) A study on the structure–function relationship of lipopeptide biosurfactants, Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids 1488, 211-218.
45. [23] Lotfabad, T., Shourian, M., Roostaazad, R., Najafabadi, A., Adelzadeh, M., and Noghabi, K. (2009) An efficient biosurfactant-producing bacterium Pseudomonas aeruginosa MR01, isolated from oil excavation areas in south of Iran, Colloids and Surfaces B: Biointerfaces 69, 183-193.
47. [24] Youssef, N., Duncan, K., Nagle, D., and Savage, K. (2004) Comparison of methods to detect biosurfactant production by diverse microorganisms, Journal of Microbiological Methods 56, 339-347.
49. [25] Plaza, G., Zjawiony, I., and Banat, I. (2006) Use of different methods for detection of thermophilic biosurfactantproducing bacteria from hydrocarbon-contaminated and bioremediated soils, Journal of Petroleum Science and Engineering 50, 71-77.
51. [26] Onbasli, D., and Aslim, B. (2009) Biosurfactant production in sugar beet molasses by some Pseudomonas spp, J. Environ. Biol. 30, 161-163.
53. [27] Gudiña, E., Fernandes, E., Rodrigues, A., Teixeira, J., and Rodrigues, L. (2015) Biosurfactant production by Bacillus subtilis using corn steep liquor as culture medium, Frontiers in Microbiology 6, 1-7.
55. [28] Smyth, T., Rudden, M., Tsaousi, K., Marchant, R., and Banat, I. (2014) Protocols for the Detection and Chemical Characterisation of Microbial Glycolipids, In Hydrocarbon and Lipid Microbiology Protocols, Springer Protocols Handbooks (McGenity, T. J., Ed.), Springer-Verlag Berlin Heidelberg.
57. [29] Yin, H., Qiang, J., Jia, Y., Ye, J., Peng, H., Qin, H., Zhang, N., and He, B. (2009) Characteristics of biosurfactant produced by Pseudomonas aeruginosa S6 isolated from oil-containing wastewater, Process Biochemistry 44, 302-308.
59. [30] Saikia, R., Deka, S., Deka, M., and Banat, I. (2012) Isolation of biosurfactant-producing Pseudomonas aeruginosa RS29 from oil-contaminated soil and evaluation of different nitrogen sources in biosurfactant production, Annals of microbiology 62, 753-763.
61. [31] Smyth, T., Perfumo, A., Marchant, R., and Banat, I. (2010) Isolation and analysis of low molecular weight microbial glycolipids, In Handbook of hydrocarbon and lipid microbiology (Timmis, K. N., Ed.), pp 3705-3723, Springer.
63. [32] Sekowska, A., Gospodarek, E., Janickca, G., Jachna-Sawicka, K., and Sawicki, M. (2005) Hydrolytic and haemolytic activity of Klebsiella pneumoniae and Klebsiella oxytoca, Medycyna doswiadczalna i mikrobiologia 58, 135-141.
65. [33] Abdel-Mawgoud, A., Hausmann, R., Lépine, F., Müller, M., and Déziel, E. (2011) Rhamnolipids: detection, analysis, biosynthesis, genetic regulation, and bioengineering of production, In Biosurfactants, pp 13-55, Springer.
67. [34] Sudhakar Babu, P., Vaidya, A., Bal, A., Kapur, R., Juwarkar, A., and Khanna, P. (1996) Kinetics of biosurfactant production by Pseudomonas aeruginosa strain BS2 from industrial wastes, Biotechnology letters 18, 263-268.
69. [35] Saimmai, A., Sobhon, V., and Maneerat, S. (2011) Molasses as a whole medium for biosurfactants production by Bacillus strains and their application, Applied biochemistry and biotechnology 165, 315-335.
71. [36] Joshi, S., Bharucha, Jha, S., Yadav, S., Nerurkar, A., and Desai, A. J. (2008) Biosurfactant production using molasses and whey under thermophilic conditions, Bioresource Technology 99, 195-199.
73. [37] Patel, R., and Desai, A. (1997) Biosurfactant production by Pseudomonas aeruginosaGS3 from molasses, Letters in Applied Microbiology 25, 91-94.
75. [38] Rashedi, H., Assadi, M. M., Bonakdarpour, B., and Jamshidi, E. (2005) Environmental importance of rhamnolipid production from molasses as a carbon source, International Journal of Environmental Science & Technology 2, 59-62.
Add your comments about this article : Your username or Email:
CAPTCHA
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.