Volume 13, Issue 2 (2023)                   JMBS 2023, 13(2): 46-70 | Back to browse issues page

‎ 20.1001.1.23222115.1401.13.2.3.8

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Farhadian S, Momeni L, Shareghi B. Interaction of cadmium telluride semiconductor nanoparticles on egg white lysozyme using spectroscopic methods and enzymatic kinetics. JMBS 2023; 13 (2) :46-70
URL: http://biot.modares.ac.ir/article-22-49910-en.html
Interaction of cadmium telluride semiconductor nanoparticles on egg white lysozyme using spectroscopic methods and enzymatic kinetics
Sadegh Farhadian1 , Lida Momeni * 2, Behzad Shareghi3
1- Assistant Professor, Department of Biology, Faculty of Science, Shahrekord University, Shahrekord, Shahrekord University, Rahbar BLV., Shahrekord
2- Assistant Professor, Department of Biology, Faculty of Science, Payam Noor University, Payam Noor University, Ferdowsi St., Shahrekord, Iran , itslida@gmail.com
3- Professor, Department of Biology, Faculty of Science, Shahrekord University, Shahrekord, Shahrekord University, Rahbar BLV., Shahrekord
Abstract:   (791 Views)
In this article, the interaction between lysozyme and CdTe nanoparticles was investigated by UV-Vis spectroscopy, fluorescence, thermal stability, kinetics, and circular dichroism (CD) spectroscopic methods at pH 7.25. It was proved that the fluorescence quenching of lysozyme by CdTe NPs was mainly a result of the formation of the CdTe–lysozyme complex. By the fluorescence quenching results, the Stern–Volmer quenching constant (KSV), binding constant (Ka), and binding sites (n) were calculated. Under pH 7.25 conditions, the level of binding constant is determined to be 2.33×103 from fluorescence data. The hydrogen bond or van der Waals force is involved in the binding process. The blue shift of the fluorescence spectral peak of protein after the addition of CdTe nanoparticles reveals that the microenvironments around tryptophan residues are disturbed by CdTe nanoparticles. The effect of CdTe NPs on the conformation of lysozyme has been analyzed by means of UV-Vis spectra and CD spectra, which provided evidence that the secondary structure of lysozyme has been changed by the interaction of CdTe NPs with lysozyme.
Keywords: Lysozyme, cadmium telluride, Fluorescence quenching, Thermal stability, Activity
Full-Text [PDF 1949 kb]   (208 Downloads)    
Article Type: Original Research | Subject: Nanotechnology
Received: 2021/02/5 | Accepted: 2022/04/3 | Published: 2023/07/10
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