Volume 9, Issue 4 (2018)
JMBS 2018, 9(4): 495-500 |
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Hashemzadeh Z, Jafarkhani Kermani M, Hamidoghli Y. Optimization of Direct Regeneration Method in Ziziphus jujuba Mill. JMBS 2018; 9 (4) :495-500
URL: http://biot.modares.ac.ir/article-22-13970-en.html
URL: http://biot.modares.ac.ir/article-22-13970-en.html
1- Horticulture Department, Agriculture Faculty, Guilan University, Guilan, Iran
2- Tissue & Cell Culture Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran, Tissue & Cell Culture Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran ,m.j.kermani@abrii.ac.ir
2- Tissue & Cell Culture Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran, Tissue & Cell Culture Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran ,
Abstract: (10244 Views)
Aims: The mass propagation and breeding new varieties of Ziziphus jujuba Mill as a valuable fruit tree and herb, which is well adapted to dry and semi-arid climate, is very important. The aim of the present research was to optimize direct regeneration method in Ziziphus jujuba Mill.
Materials and Methods: In this experimental study, the explants consisted of leaf cut into 3 parts, leaf cut from 4 sides, and full leaf of in vitro and compared in Murashige and Skoog and woody plant media with different concentrations of Thidiazuron (TDZ; 0, 5, 10, 15, and 20μM) and Naphthalene acetic acid (NAA, 0 and 1μM). The effect of 2 and 4 weeks of darkness on regeneration rate was investigated. The experiments were conducted in factorial based on a completely randomized design. The mean of statistical data was compared using Duncan’s multiple range test. SAS 9.3.1 software was used and the difference was considered significant at 1% probability level.
Findings: The 2 weeks of darkness treatment with the mean of 1.38 was better than the 4 weeks of darkness treatment. The Maximum number of shoots (2.27) was obtained in leaf cut into 3 parts. The maximum percent of regeneration (75.0%) and highest number of regenerated shoots (4.83) were obtained in the MS medium containing 10μΜ TDZ and 1μΜ NAA.
Conclusion: Regeneration rates in Ziziphus jujuba Mill is affected by the type of explant, culture media and plant growth regulators. Maximum rate of regeneration is observed in leaf cut into 3 parts and cultured on MS medium containing 1μM NAA and 10μΜ TDZ. Plantlets are rooted and successfully acclimatized at “in vivo” conditions.
Materials and Methods: In this experimental study, the explants consisted of leaf cut into 3 parts, leaf cut from 4 sides, and full leaf of in vitro and compared in Murashige and Skoog and woody plant media with different concentrations of Thidiazuron (TDZ; 0, 5, 10, 15, and 20μM) and Naphthalene acetic acid (NAA, 0 and 1μM). The effect of 2 and 4 weeks of darkness on regeneration rate was investigated. The experiments were conducted in factorial based on a completely randomized design. The mean of statistical data was compared using Duncan’s multiple range test. SAS 9.3.1 software was used and the difference was considered significant at 1% probability level.
Findings: The 2 weeks of darkness treatment with the mean of 1.38 was better than the 4 weeks of darkness treatment. The Maximum number of shoots (2.27) was obtained in leaf cut into 3 parts. The maximum percent of regeneration (75.0%) and highest number of regenerated shoots (4.83) were obtained in the MS medium containing 10μΜ TDZ and 1μΜ NAA.
Conclusion: Regeneration rates in Ziziphus jujuba Mill is affected by the type of explant, culture media and plant growth regulators. Maximum rate of regeneration is observed in leaf cut into 3 parts and cultured on MS medium containing 1μM NAA and 10μΜ TDZ. Plantlets are rooted and successfully acclimatized at “in vivo” conditions.
Article Type: Research Paper |
Subject:
Agricultural Biotechnology
Received: 2016/07/23 | Accepted: 2018/02/21 | Published: 2018/12/21
Received: 2016/07/23 | Accepted: 2018/02/21 | Published: 2018/12/21
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