Volume 9, Issue 4 (2018)
JMBS 2018, 9(4): 635-641 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Zamani F, Merati A, Latifi M, Ghanbari Alanagh H, Nadipour F. Effect of Plasma Treatment on Cell Culture in Poly Lactic Glycolic Acid Nanofibrous Scaffold. JMBS 2018; 9 (4) :635-641
URL: http://biot.modares.ac.ir/article-22-14426-en.html
URL: http://biot.modares.ac.ir/article-22-14426-en.html
1- Hazrat-e Masoumeh University, Qom, Iran
2- “Advanced Textile Materials & Technologies Research Institute” and “Nano-fibrous Structures Department, Textile Engineering Faculty”, Amirkabir University of Technology, Tehran, Iran, Textile Engineering Faculty, Amirkabir University of Technology, NO. 424, Hafez Street, Tehran, Iran. Postal Code: 1591634311 ,merati@aut.ac.ir
3- Nano-fibrous Structures Department, Textile Engineering Faculty, Amirkabir University of Technology, Tehran, Iran
4- Medical Nanotechnology Department, Advanced Technologies in Medicine Faculty, Tehran University of Medical Science, Tehran, Iran
2- “Advanced Textile Materials & Technologies Research Institute” and “Nano-fibrous Structures Department, Textile Engineering Faculty”, Amirkabir University of Technology, Tehran, Iran, Textile Engineering Faculty, Amirkabir University of Technology, NO. 424, Hafez Street, Tehran, Iran. Postal Code: 1591634311 ,
3- Nano-fibrous Structures Department, Textile Engineering Faculty, Amirkabir University of Technology, Tehran, Iran
4- Medical Nanotechnology Department, Advanced Technologies in Medicine Faculty, Tehran University of Medical Science, Tehran, Iran
Abstract: (4484 Views)
Aims: Tissue engineering and replacement of damaged tissue in medical science is very important and more effective than person-to-person transplantation. Therefore, the production of scaffolds from natural and synthetic polymers with desirable properties to reproduce damaged tissues is increasing. The aim of the present study was to investigate the effect of plasma treatment on contact angle or hydrophilicity of poly-lactic glycolic acid nanofibrous scaffolds and cell culture efficiency.
Materials and Methods: In the present experimental research, two types of solvents such as pure chloroform and the choloroform80% and dimethyl formaldehyde20% were used for electrospinning solution. The level of electrospun scaffolds was corrected by plasma technology; then, the African green monkey kidney (VERO) cells were cultured on them. The raw or non-treated electrospun scaffold was compared with that of plasma treated in hydrophilicity and cell culture viewpoints. To compare the hydrophilicity of scaffolds, the contact angle of them was measured.
Findings: The samples treated with plasma show lower contact angle and consequently higher hydrophilicity. C=O and C-O groups increased in the plasma-treated samples in comparison with those of raw samples. Plasma scaffold level correction improved the adhesion, growth, and proliferation of cells compared to non-treated scaffolds.
Conclusion: The contact angle of the plasma-treated samples is significantly reduced. Plasma treatment can increase the hydrophilicity of poly-lactic glycolic acid nanofibrous scaffolds, and cell adhesion and growth on plasma-treated scaffolds is better than cell growth and proliferation on non-treated scaffolds.
Materials and Methods: In the present experimental research, two types of solvents such as pure chloroform and the choloroform80% and dimethyl formaldehyde20% were used for electrospinning solution. The level of electrospun scaffolds was corrected by plasma technology; then, the African green monkey kidney (VERO) cells were cultured on them. The raw or non-treated electrospun scaffold was compared with that of plasma treated in hydrophilicity and cell culture viewpoints. To compare the hydrophilicity of scaffolds, the contact angle of them was measured.
Findings: The samples treated with plasma show lower contact angle and consequently higher hydrophilicity. C=O and C-O groups increased in the plasma-treated samples in comparison with those of raw samples. Plasma scaffold level correction improved the adhesion, growth, and proliferation of cells compared to non-treated scaffolds.
Conclusion: The contact angle of the plasma-treated samples is significantly reduced. Plasma treatment can increase the hydrophilicity of poly-lactic glycolic acid nanofibrous scaffolds, and cell adhesion and growth on plasma-treated scaffolds is better than cell growth and proliferation on non-treated scaffolds.
Article Type: _ |
Subject:
Agricultural Biotechnology
Received: 2017/01/22 | Accepted: 2017/09/6 | Published: 2018/12/21
Received: 2017/01/22 | Accepted: 2017/09/6 | Published: 2018/12/21
References
1. Gholipour-Kanani A, Bahrami H, Joghataie MT, Samadikuchaksaraei A. Nanofibrous scaffolds based on poly (caprolactone)/chitosan/poly (vinyl alcohol) blend for skin tissue engineering. Iran J Polymer Sci Technol. 2013;26(2):159-70. [Persian] [Link]
2. Gholipour-Kanani A, Bahrami H, Joghataie M, Samadikuchaksaraei A, Ahmadi-Taftie H, Rabbani S, et al. Tissue engineered poly (caprolactone)-chitosan-poly (vinyl alcohol) nanofibrous scaffolds for burn and cutting wound healing, IET Nanobiotechnol. 2014;8(2):123-31. [Link] [DOI:10.1049/iet-nbt.2012.0050]
3. Ghasemi-Mobarakeh L, Prabhakaran MP, Morshed M, Nasr-Esfahani MH, Ramakrishna S. Electrospun poly (ɛ-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering. Biomaterials. 2008;29(34):4532-9. [Link] [DOI:10.1016/j.biomaterials.2008.08.007]
4. Jahanmard-Hoseinabadi F, Amani-Tehran M, Zamani F, Nematollahi M, Ghasemi-Mobarakeh L, Nasr-Esfahani MH. Effect of nanoporous fibers on growth and proliferation of cells on electrospun poly (ϵ-caprolactone) scaffolds. Int J Polymeric Bio. 2013;63(2):57-64. [Link]
5. Zamani F, Amani-Tehran M, Latifi M, Shokrgozar MA. The influence of surface nanoroughness of electrospun PLGA nanofibrous scaffold on nerve cell adhesion and proliferation. J Mater Sci Mater Med. 2013;24(6):1551-60. [Link] [DOI:10.1007/s10856-013-4905-6]
6. Yalcinkaya F, Yalcinkaya B, Pazourek A, Mullerova J, Stuchlik M, Maryska J. Surface modification of electrospun PVDF/PAN nanofibrous layers by low vacuum plasma treatment. 2016;2016:Article ID 4671658, 9 pages. [Link]
7. Abbasi N, Soudi S, Hayati-Roodbari N, Dodel M, Soleimani M. The effects of plasma treated electrospun nanofibrous poly (ε-caprolactone) scaffolds with different orientations on mouse embryonic stem cell proliferation. Cell J. 2014;16(3):245-54. [Link]
8. Pappa AM, Karagkiozaki V, Krol S, Kassavetis S, Konstantinou D, Pitsalidis C, et al. Oxygen-plasma-modified biomimetic nanofibrous scaffolds for enhanced compatibility of cardiovascular implants. Beilstein J Nanotechnol. 2015;6:254-62 [Link] [DOI:10.3762/bjnano.6.24]
9. Bacakova M, Lopot F, Hadraba D, Varga M, Zaloudkova M, Stranska D, et al. Effects of fiber density and plasma modification of nanofibrous membranes on the adhesion and growth of HaCaT keratinocytes. J Biomater Appl. 2015;29(6):837-53. [Link] [DOI:10.1177/0885328214546647]
10. Desai TA. Micro- and nanoscale structures for tissue engineering constructs. Med Eng Phys. 2000;22(9):595-606. [Link] [DOI:10.1016/S1350-4533(00)00087-4]
11. Sill TJ, von Recum HA. Electrospinning: Applications in drug delivery and tissue engineering. Biomaterials. 2008;29(13):1989-2006. [Link] [DOI:10.1016/j.biomaterials.2008.01.011]
12. Solouk A, Brian GC, Mirzadeh H, Seifalian MA. Application of plasma surface modification techniques to improve hemocompatibility of vascular grafts: A review. Biotechnol Appl Biochem. 2011;58(5):311-27. [Link] [DOI:10.1002/bab.50]
13. Safinia L, Datan N, Höhse M, Mantalaris A, Bismarck A. Towards a methodology for the effective surface modification of porous polymer scaffold. Biomaterials. 2005;26(36):7537-47. [Link] [DOI:10.1016/j.biomaterials.2005.05.078]
14. Khoshdel N, Mahboobi F. Comparison of Nitrogen-Carbonation processes by active touring and the common method of Low-alloy Steel DIN 1/6582. Seminar on Surface Engineering and Heat Treatment. Tehran: Iranian Society of Science and Technology; 2006. [Link]
15. Shishoo R. Plasma technologies for textiles. 1st edition. Amsterdam: Elsevier; 2007. [Link] [DOI:10.1533/9781845692575]
16. Morent R, Nathalie DG, Tim D, Peter D, Christophe L. Plasma surface modification of biodegradable polymers: A review. Plasma Proc Polym. 2011;8(3):171-90. [Link] [DOI:10.1002/ppap.201000153]
17. Hasirci N, Endogan T, Vardar E, Kiziltay A, Hasirci V. Effect of oxygen plasma on surface properties and biocompatibility of PLGA films. Surf Inter Anal. 2010;42(6-7):486-91. [Link] [DOI:10.1002/sia.3247]
18. Khorasani MT, Mirzadeh H. Effect of oxygen plasma treatment on surface charge and wettability of PVC blood bag—In vitro assay. Radi Physics Chem. 2006;76(6):1011-6. [Link] [DOI:10.1016/j.radphyschem.2006.10.002]
19. Wan Y, Qu X, Lu J, Zhu C, Wan L, Yang J, et al. Characterization of surface property of poly (lactide-co-glycolide) after oxygen plasma treatment. Biomaterials. 2004;25(19):4777-83. [Link] [DOI:10.1016/j.biomaterials.2003.11.051]
20. Govorkova EA, Murti G, Meignier B, Taisne CD, Webster RG. African green monkey kidney (vero) cells provide an alternative host cell system for influenza A and B viruses. J Virol. 1996;70(8):5519-24. [Link]
21. Ramakrishna S. An introduction to electrospinning and nanofibers. Singapore: World Scientific; 2004. [Link]
22. Pavia D, Lompan G, Kariz J. Attitude on spectroscopy. Movasagh B, translator. Tehran: Elmi & Fani Press; 1993. [Persian] [Link]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |