Volume 13, Issue 1 (2022)                   JMBS 2022, 13(1): 1-14 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Arab S S, Hassan Sajedi R, Behmanesh M, sepehri S. Histidine-tagging effect on TGF-β molecular dynamics. JMBS 2022; 13 (1) :1-14
URL: http://biot.modares.ac.ir/article-22-17158-en.html
1- Tarbiat Modares University, Tehran
2- Tarbiat Modares university, Tehran
3- Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:   (1596 Views)
Transforming growth factor beta (TGF-β), is a small homodimeric signaling protein. The TGF-β isoforms (TGFβ1, β2 and β3) are involved in many cellular processes including growth inhibition, extracellular matrix remodeling, tissue development, cell migration, invasion and immune regulation. For research aims, TGFβs are overexpressed using recombinant eukaryotic cell or bacterial expression systems. For achieving an efficient purification of TGF-β by immobilized metal ion affinity chromatography (IMAC), a histidine tag was placed either at the C-terminal (C-TGFβ) or N-terminal (N-TGFβ) region of the sequence and the effect of His-tag on TGF-β structure has been studied by computational tools. Proteins 3D structures were modeled using MODELLER software and molecular dynamics simulation of native TGF-β and modelled proteins, N-TGFβ and C-TGFβ were studied in water by GROMACS package. Protein dynamics modeling indicated that the His-tag attached at the C-terminus but not at the N-terminus of the TGF-β can affect the fluctuations of amino acids and protein structure. It is concluded that the C-terminal tagging may cause distortion and misfolding in the structure.
Full-Text [PDF 901 kb]   (939 Downloads)    
Subject: Agricultural Biotechnology
Received: 2018/03/1 | Accepted: 2021/07/1 | Published: 2023/01/4

Add your comments about this article : Your username or Email:

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.