Volume 10, Issue 4 (2019)                   JMBS 2019, 10(4): 635-646 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bakhshian Nik A, Vahidi B. Simulation of the Effects of Shear Flow of the Culture Medium Fluid on Stem Cells using the Scaffolds of Hard Tissue Engineering. JMBS 2019; 10 (4) :635-646
URL: http://biot.modares.ac.ir/article-22-21919-en.html
1- Life Science Engineering Department, New Sciences and Technologies Faculty, University of Tehran, Tehran, Iran
2- Life Science Engineering Department, New Sciences and Technologies Faculty, University of Tehran, Tehran, Iran, Science & Technology Faculty, North Kargar Street, Tehran, Iran. Postal Code: 1439957131 , bahman.vahidi@ut.ac.ir
Abstract:   (3419 Views)
Aims: In bone tissue engineering, the scaffold as a supportive structure, plays a vital role. Putting the scaffold in dynamic cell culture, such as perfusion bioreactor, makes the role of mechanical parameters such as shear stress and hydrodynamic pressure more important. On the other hand, these mechanical parameters are influenced by scaffold architecture. In this study, the effects of bone scaffold architecture on mechanical stimuli have been analyzed and their effects on the mesenchymal stem cell fate have been predicted.
Material & Methods: Using the tools of computer simulation, five bone scaffolds (Gyroid, high porous Gyroid, Diamond, IWP, and gradient architecture Gyroid) based on mathematical functions of minimal surfaces were designed and exposed in a simulated dynamic cell culture under the inlet velocities of 1, 10, 25, 50, and 100μm/s. Cell accumulation on the inner part of the scaffold was considered as an 8.5-micron layer. This layer was designed for Gyroid and IWP scaffolds.
Findings: Based on the results, Diamond scaffold showed the most efficient performance from the homogeneity of stresses point of view. In the presence of the cell layer, the von Mises stress was reported as 60 and 50 mPa on the Gyroid and IWP scaffolds, respectively which eases osteogenic differentiation.
Conclusion: In gradient architecture scaffolds under dynamic conditions, there is a gradient in shear stress that causes various signaling in different positions of theses scaffold and facilitates multi-differentiation of the cells on the same scaffold.
Full-Text [PDF 1282 kb]   (952 Downloads)    
Article Type: Original Research | Subject: Biological system
Received: 2018/06/26 | Accepted: 2019/06/12 | Published: 2019/12/21

1. Subramony SD, Su A, Yeager K, Lu HH. Combined effects of chemical priming and mechanical stimulation on mesenchymal stem cell differentiation on nanofiber scaffolds. J Biomech. 2014;47(9):2189-96. [Link] [DOI:10.1016/j.jbiomech.2013.10.016]
2. Nik AB, Vahidi B. The effect of bone scaffold gradient architecture design on stem cell mechanical modulation: A computational study. 22nd Iranian Conference on Biomedical Engineering (ICBME), 25-27 November, 2015, Tehran, Iran. Piscataway: IEEE; 2015. pp. 309-13. [Link]
3. Bakhshian Nik A, Vahidi B, Moradkhani M. Effects of scaffold architecture on efficiency of mechanical stimulations of mesenchymal stem cells under fluid flow. 11th Congress on Stem Cell Biology & Technology. Royan International Twin Congress on Reproductive Biomedicine and Stem Cells Biology & Technology; 2015 Sep 2-4, Iran: Tehran. [Persian] [Link]
4. Wu Sh, Liu X, Yeung KW, Liu Ch, Yang X. Biomimetic porous scaffolds for bone tissue engineering. Mater Sci Eng R Rep. 2014;80:1-36. [Link] [DOI:10.1016/j.mser.2014.04.001]
5. Giannitelli SM, Accoto D, Trombetta M, Rainer A. Current trends in the design of scaffolds for computer-aided tissue engineering. Acta Biomater. 2014;10(2):580-94. [Link] [DOI:10.1016/j.actbio.2013.10.024]
6. Karande TS, Ong JL, Agrawal CM. Diffusion in musculoskeletal tissue engineering scaffolds: Design issues related to porosity, permeability, architecture, and nutrient mixing. Annal Biomed Eng. 2004;32(12):1728-43. [Link] [DOI:10.1007/s10439-004-7825-2]
7. Yeatts AB, Choquette DT, Fisher JP. Bioreactors to influence stem cell fate: Augmentation of mesenchymal stem cell signaling pathways via dynamic culture systems. Biochimica Biophys Acta. 2013;1830(2):2470-80. [Link] [DOI:10.1016/j.bbagen.2012.06.007]
8. Dabagh M, Jalali P, Butler PJ, Tarbell JM. Shear-induced force transmission in a multicomponent, multicell model of the endothelium. J R Soc Interface. 2014;11(98):20140431. [Link] [DOI:10.1098/rsif.2014.0431]
9. Will J, Detsch R, Boccaccini AR. Structural and biological characterization of scaffolds. In: Bandyopadhyay A, Bose S, editors. Characterization of biomaterials. Amsterdam: Elsevier; 2013. pp. 299-310. [Link] [DOI:10.1016/B978-0-12-415800-9.00008-5]
10. Chen Y, Schellekens M, Zhou Sh, Cadman J, Li W, Appleyard R, et al. Design optimization of scaffold microstructures using wall shear stress criterion towards regulated flow-induced erosion. J Biomech Eng. 2011;133(8):081008. [Link] [DOI:10.1115/1.4004918]
11. Melchels FP, Bertoldi K, Gabbrielli R, Velders AH, Feijen J, Grijpma DW. Mathematically defined tissue engineering scaffold architectures prepared by stereolithography. Biomaterials. 2010;31(27):6909-16. [Link] [DOI:10.1016/j.biomaterials.2010.05.068]
12. Abbasi F, Ghanian MH, Baharvand H, Vahidi B, Eslaminejad MB. Engineering mesenchymal stem cell spheroids by incorporation of mechanoregulator microparticles. J Mech Behav Biomed Mater. 2018;84:74-87. [Link] [DOI:10.1016/j.jmbbm.2018.04.026]
13. Vaez Ghaemi R, Vahidi B, Sabour MH, Haghighipour N, Alihemmati Z. Fluid-structure interactions analysis of shear‐induced modulation of a mesenchymal stem cell: An image‐based study. Artif Organs. 2016;40(3):278-87. [Link] [DOI:10.1111/aor.12547]
14. Alihemmati Z, Vahidi B, Haghighipour N, Salehi M. Computational simulation of static/cyclic cell stimulations to investigate mechanical modulation of an individual mesenchymal stem cell using confocal microscopy. Mater Sci Eng C. 2017;70(Pt 1):494-504. [Link] [DOI:10.1016/j.msec.2016.09.026]
15. Yoo DJ. Computer-aided porous scaffold design for tissue engineering using triply periodic minimal surfaces. Int J Precis Eng Manuf. 2011;12(1):61-71. [Link] [DOI:10.1007/s12541-011-0008-9]
16. Gibson LJ, Ashby MF. Cellular solids: Structure and properties. 2nd Edition. Cambridge: Cambridge University Press; 1999. [Link]
17. Van De Velde K, Kiekens P. Biopolymers: Overview of several properties and consequences on their applications. Polym Test. 2002;21(4):433-42. [Link] [DOI:10.1016/S0142-9418(01)00107-6]
18. Woodruff MA, Hutmacher DW. The return of a forgotten polymer-polycaprolactone in the 21st century. Prog Polym Sci. 2010;35(10):1217-56. [Link] [DOI:10.1016/j.progpolymsci.2010.04.002]
19. Eshraghi Sh, Das S. Mechanical and microstructural properties of polycaprolactone scaffolds with one-dimensional, two-dimensional, and three-dimensional orthogonally oriented porous architectures produced by selective laser sintering. Acta Biomater. 2010;6(7):2467-76. [Link] [DOI:10.1016/j.actbio.2010.02.002]
20. Zhao F, Vaughan TJ, Mcnamara LM. Multiscale fluid-structure interaction modelling to determine the mechanical stimulation of bone cells in a tissue engineered scaffold. Biomech Model Mechanobiol. 2015;14(2):231-43. [Link] [DOI:10.1007/s10237-014-0599-z]
21. Yu H, Tay CY, Leong WS, Tan SC, Liao K, Tan LP. Mechanical behavior of human mesenchymal stem cells during adipogenic and osteogenic differentiation. Biochem Biophys Res Commun. 2010;393(1):150-5. [Link] [DOI:10.1016/j.bbrc.2010.01.107]
22. Zeng X, Li Sh. Multiscale modeling and simulation of soft adhesion and contact of stem cells. J Mech Behav Biomed Mater. 2011;4(2):180-9. [Link] [DOI:10.1016/j.jmbbm.2010.06.002]
23. Ribeiro AJ, Tottey S, Taylor RW, Bise R, Kanade T, Badylak SF, et al. Mechanical characterization of adult stem cells from bone marrow and perivascular niches. J Biomech. 2012;45(7):1280-7. [Link] [DOI:10.1016/j.jbiomech.2012.01.032]
24. Sengers BG, Taylor M, Please CP, Oreffo RO. Computational modelling of cell spreading and tissue regeneration in porous scaffolds. Biomaterials. 2007;28(10):1926-40. [Link] [DOI:10.1016/j.biomaterials.2006.12.008]
25. Blecha LD, Rakotomanana L, Razafimahéry F, Terrier A, Pioletti DP. Mechanical interaction between cells and fluid for bone tissue engineering scaffold: Modulation of the interfacial shear stress. J Biomech. 2010;43(5):933-7. [Link] [DOI:10.1016/j.jbiomech.2009.11.004]
26. Norato JA, Wagoner Johnson AJ. A computational and cellular solids approach to the stiffness-based design of bone scaffolds. J Biomech Eng. 2011;133(9):091003. [Link] [DOI:10.1115/1.4004994]
27. Yang Y, El Haj A. Enhancement of mechanical signals for tissue engineering bone. In: Ashammakhi N, Reis RL, editors. 2nd Volume. Topics in Tissue Engineering. Oulu: University of Oulu; 2005. [Link]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.