Volume 9, Issue 4 (2018)                   JMBS 2018, 9(4): 501-506 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Soleimani S, Yousefzadi M, Rezadoost H. Quantitative and Qualitative Identification of Polyhydroxylated Naphthoquinone Pigments from Shell and Spine of Echinometra Mathaei of the Persian Gulf. JMBS 2018; 9 (4) :501-506
URL: http://biot.modares.ac.ir/article-22-24437-en.html
1- Marine Biology Department, Marine Science & Technology Faculty, University of Hormozgan, Bandar Abbas, Iran
2- Marine Biology Department, Marine Science & Technology Faculty, University of Hormozgan, Bandar Abbas, Iran, University of Hormozgan, Kilometer 9 of Minab Road, Bandar Abbas, Hormozgan Province, Iran. , morteza110110@gmail.com
3- Phytochemistry Department, Plants & Pharmaceutical Ingredients Institute, Shahid Baheshti University, Bandar Abbas, Iran
Abstract:   (8615 Views)
Aims: Sea urchins have been extensively studied due to the commercial importance of their gonads in the global industry. Although after removal of the edible gonads, the remaining shell and spines are usually discarded, they are known to possess various polyhydroxylated naphthoquinone (PHNQ) pigments. The aim of the present research was quantitative and qualitative identification of PHNQ pigments from shell and spine of Echinometra Mathaei of the Persian Gulf.
Materials and Methods: In this experimental study, the Echinometra mathaei was used as the sea urchin test sample. Sea urchins were collected in 2013 from Zeytoon Park in Qeshm Island, Persian Gulf. Shell and spine pigments were extracted by hydrochloric acid from sea urchin. Then, the quantity of Naphthoquinone compounds was evaluated by spectrophotometric and their quality was evaluated by Liquid chromatography–mass spectrometry (LC-MS) and High-performance liquid chromatography (HPLC). The data were analysed by ANOVA and Duncan's new multiple range test at 5% probability level, using SPSS 19 software and the diagrams were drawn by Excel 2013 software.
Findings: The most pigments were Spinochrome A, C, B, and Echinochrome A, respectively. The presence of PHNQ pigments were confirmed in pigments Spinochrome B and C, Echinochrome A, and Spinochrome A, respectively.
Conclusion: The presence of each of the four pigments in shell and spine pigments is confirmed by quantitative and qualitative methods. The most pigments are Spinochrome A, C, B, and Echinochrome A, respectively.
Full-Text [PDF 555 kb]   (2851 Downloads)    
Subject: Agricultural Biotechnology
Received: 2016/10/5 | Accepted: 2017/02/20 | Published: 2018/12/21

References
1. Bragadeeswaran S, Sri Kumaran N, Prasath Sankar P, Prabahar R. Bioactive potential of sea urchin Temnopleurus toreumaticus from Devanampattinam, Southeast coast of India. J Pharm Altern Med. 2013;2(3):9-18. [Link]
2. Jimeno J, Faircloth G, Sousa-Faro JM, Scheuer P, Rinehart K. New marine derived anticancer therapeutics- a journey from the sea to clinical trials. Mar Drugs. 2004;2(1):14-29. [Link] [DOI:10.3390/md201014]
3. Bodnar A. Proteomic profiles reveal age-related changes in coelomic fluid of sea urchin species with different life spans. Exp Gerontol. 2013;48(5):525-30. [Link] [DOI:10.1016/j.exger.2013.01.014]
4. Khaleghi M, Safahieh A, Savari A, Doustshenas B, Owfi F. The process of long and location changes of sea star (Astropecten hemprichi) and sea urchin (Diadema setosum) in the coast of Chabahar Gulf. J Mar Sci Tech. 2012;11(3):81-90. [Link]
5. Vadas Sr RL, Beal B, Dowling T, Fegley JC. Experimental field tests of natural algal diets on gonad index and quality in the green sea urchin, Strongylocentrotus droebachiensis: a case for rapid summer production in post-spawned animals. Aquaculture. 2000;182(1-2):115-35. [Link] [DOI:10.1016/S0044-8486(99)00254-9]
6. Amarowicz R, Synowiecki J, Shahidi F. Chemical composition of shells from red (Strongylocentrotus franciscanus) and green (Strongylocentrotus droebachiensis) sea urchin. Food Chem. 2012;133(3):822-6. [Link] [DOI:10.1016/j.foodchem.2012.01.099]
7. Zhou DY, Qin L, Zhu BW, Wang XD, Tan H, Yang JF, et al. Extraction and antioxidant property of polyhydroxylated naphthoquinone pigments from spines of purple sea urchin Strongylocentrotus nudus. Food Chem. 2011;129(4):1591-7. [Link] [DOI:10.1016/j.foodchem.2011.06.014]
8. Kuwahara R, Hatate H, Chikami A, Murata H, Kijidani Y. Quantitative separation of antioxidant pigments in purple sea urchin shells using a reversed-phase high performance liquid chromatography. LWT Food Sci Technol. 2010;43(8):1185-90. [Link] [DOI:10.1016/j.lwt.2010.03.005]
9. Anderson HA, Mathieson JW, Thomson RH. Distribution of Spinochrome pigments in echinoids. Comp. Biochem. Physiol. 1969;28(1):333-45. [Link] [DOI:10.1016/0010-406X(69)91347-4]
10. Soleimani S, Yousefzadi M, Moein S, Amrollahi Bioki N, Keshavarz M, Aslian H. Evaluation of antioxidant activity and polyphenolic content of sea urchin Echinometra mathaei from the Persian Gulf. Biotechnol Tarbiat Modares Univ. 2015;6(2):70-80 [Persian]. [Link]
11. Kuwahara R, Hatate H, Yuki T, and et al. Antioxidant property of polyhydroxylated naphthoquinone pigments from shells of purple sea urchin Anthocidaris crassispina. LWT Food Sci. Technol. 2009;42(7):1296-1300. [Link] [DOI:10.1016/j.lwt.2009.02.020]
12. Price AR. Studies on the echinoderm fauna of the western Arabian Gulf. J Nat Hist. 1981;15(1):1-15. [Link] [DOI:10.1080/00222938100770011]
13. Coles SL, McCain JC. Environmental factors affecting benthic infaunal communities of the Western Arabian Gulf. Mar. Environ. Res. 1990;29(4):289-315. [Link] [DOI:10.1016/0141-1136(90)90024-I]
14. Powell C, Hughes AD, Kelly MS, Conner S, McDougall GJ. Extraction and identification of antioxidant polyhydroxy naphthoquinone pigments from the sea urchin, Psammechinus miliaris. LWT Food Sci. Technol. 2014;59(1):455-60. [Link] [DOI:10.1016/j.lwt.2014.05.016]
15. Shikov AN, Ossipov VI, Martiskainen O, Pozharitskaya ON, Ivanova SA, Makarov VG. The offline combination of thin-layer chromatography and high-performance liquid chromatography with diode array detection and micrOTOF-Q mass spectrometry for the separation and identification of spinochromes from sea urchin (Strongylocentrotus droebachiensis) shells. J Chromatogr A. 2011;1218(50)9111-4. [Link] [DOI:10.1016/j.chroma.2011.10.045]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.