Volume 10, Issue 3 (2019)
JMBS 2019, 10(3): 465-471 |
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Zaghian S, Tohidi Moghadam T, Behmanesh M. Toxicity of Peptide-Functionalized Gold Nanorods in Hela Cell Line. JMBS 2019; 10 (3) :465-471
URL: http://biot.modares.ac.ir/article-22-25413-en.html
URL: http://biot.modares.ac.ir/article-22-25413-en.html
1- Nanobiotechnology Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran
2- Nanobiotechnology Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran, Tarbiat Modares University, Nasr Bridge, Jalal-Al-Ahmad Highway, Tehran, Iran. ,behmanesh@modares.ac.ir
2- Nanobiotechnology Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran, Tarbiat Modares University, Nasr Bridge, Jalal-Al-Ahmad Highway, Tehran, Iran. ,
Abstract: (4382 Views)
The unique physicochemical properties of nanoscale plasmonic materials have attracted considerable attention in the fabrication of hybrid nano-bio structures because of their promising applications in biosensing, imaging, and controlled-release drug delivery. The purpose of this study was the synthesis of functionalized gold nanorods (GNRs) to both reduce the toxicity and increase the biocompatibility for further applications such as the design of a therapeutic nanocarrier for nucleic acid delivery to cancerous cells. In this study, GNRs were prepared by seed-mediated method and their surface was modified by polystyrene sulfonate (PSS) polymer. Then, peptide-functionalized GNRs was fabricated via ligand exchange method through the Au-S bond. The CTAB-GNRs and functionalized nanostructures were characterized using ultraviolet-visible spectrophotometry, transmission electron microscopy (TEM), and zeta potential measurement. Finally, the cytotoxicity effects of functionalized GNRs on Hela cells were studied by MTT assay. The optimal concentration of PSS and peptide, which did not cause any aggregation and morphological perturbations of the nanostructure were obtained 50μM and 1mM respectively. The survival percentage of treated Hela cells significantly increased by surface modification of GNRs with PSS and functionalization with peptide compared to CTAB-GNRs. While LC50 of functionalized GNRs was calculated 50nM, treated cells with the same concentrations of CTABGNRs survived less than 20%. Functionalization of GNRs increases its biocompatibility and improves applications of this nanostructure as a therapeutic carrier in cancerous cells.
Article Type: Brief Communication |
Subject:
Nanotechnology
Received: 2018/09/24 | Accepted: 2018/11/11 | Published: 2019/09/21
Received: 2018/09/24 | Accepted: 2018/11/11 | Published: 2019/09/21
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