Volume 11, Issue 4 (2020)                   JMBS 2020, 11(4): 75-87 | Back to browse issues page

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Pahlavan Yali M, hjalili H, Noroozi M, Morady Y, Hallajisani A. Enhancement of native Aurantiochytrium sp.shy growth by medium optimization with response surface methodology. JMBS 2020; 11 (4) :75-87
URL: http://biot.modares.ac.ir/article-22-27990-en.html
1- seafood processing at the Iranian Fisheries Sciences Research Institute
2- Department of life science engineering, Faculty of new sciences and technologies, University of Tehran , hjalili@ut.ac.ir
3- Biotechnological Dep. faculty of Biological sciences, Alzahra University
4- Seafood Processing & Biotechnological Dep. Iranian Fisheries Sciences Research Institute
5- Caspian Faculty of Engineering, School of Engineering, University of Tehran
Abstract:   (1036 Views)
The microalgal strain Aurantiochytrium sp. shy contains considerable amounts of poly-unsaturated fatty acids (PUFAs), chiefly docosahexaenoic acid (DHA) with potential pharmaceutical and health-related attributes. Effects of various concentrations of glucose, meat extract, monosodium glutamate and sea salt on the algal biomass and DHA production have been investigated in this study. Maximum algal biomass (7.1 g/l) was obtained when the culture medium contained 60 g/l of glucose, 6 g/l of meat extract, 6 g/l monosodium glutamate and sea salt at 25ppt. Lipid contents of the alga exceeded 30% of its dry cell weight, with palmitic acid and DHA as the most abundant components. When the effect of a single additive was concerned, meat extract was significantly effective, while interaction between meat extract and glucose was the most effective in comparison with other interactions (P < 0.0001). According to the results, glucose can assure more algal and fatty acids production when adequate amounts of meat extract exist in the culture medium. Optimal results attained when the ratios of glucose to meat extract and C/N concentrations were 10 and 5, respectively. Due to its remarkable growth rate and the capability to produce substantial quantities of biomass and fatty acids, Aurantiochytrium sp.shy was found to be a major source of the beneficial ingredients, whose productivity can magnify if its culture conditions is optimized using favorable blend of growth-promoting materials.
 
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Article Type: Original Research | Subject: Industrial Biotechnology
Received: 2018/12/9 | Accepted: 2020/10/27 | Published: 2020/11/30

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