Volume 10, Issue 4 (2019)                   JMBS 2019, 10(4): 601-608 | Back to browse issues page

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Mortazavi Farsani S, Sadeghizadeh M, Shirzad H, Najafi F. The effects of Nanocurcumin on Expression Induction of Transcription Factors Involved in Hematopoietic Stem Cell Differentiation to Precursor Myeloid Cells. JMBS 2019; 10 (4) :601-608
URL: http://biot.modares.ac.ir/article-22-28278-en.html
1- Genetics Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran
2- Genetics Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran, Tarbiat Modares University, Nasr Bridge, Jalal-Al-Ahmad Highway, Tehran, Iran. Postal Code: 1411713116 , sadeghma@modares.ac.ir
3- Resin & Additives Department, Institute for Color Science & Technology, Tehran, Iran.
Abstract:   (3348 Views)
Aims: Hematopoietic stem cells are responsible for the production of blood cells in the bone marrow. During the process of differentiation, these cells commitment to two precursor cell lines include myeloid and lymphoid cells. Various blood cells, excluded lymphocytes, generates from myeloid cells. Some patients with severe anemia or thrombocytopenia receive hematopoietic stem cell through transplantation. Finding a potential component for inducing differentiation of hematopoietic stem cells before transplantation, could be an appropriate strategy for the acceleration of blood cells production in recipient persons. Various studies indicate the ability of Curcumin for inducing of cell differentiation. This component can alter many of cellular mechanisms.
Material and methods: The aim of this project was to evaluate the effects of Nanocurcumin on mRNA expression levels of GATA1, GATA2, c-Myb and Hhex genes and alteration of cellular ROS in umbilical cord blood-derived hematopoietic stem cells. Nanocurcumin was synthesized from Curcumin, Oleic acid, and PEG400. The rate of Nanocurcumin delivery into the cells was also evaluated.
Findings: Our results show that intracellular ROS and expression levels of GATA1, c-Myb, and Hhex transcription factors were significantly increased after treatment with Nanocurcumin (p<0.05). These transcription factors involve in myeloid differentiation.
Conclusion: Enhancement of these transcription factors expression making Nanocurcumin a potential candidate for applying in myeloid differentiation media and basic and clinical studies.
Full-Text [PDF 1055 kb]   (944 Downloads)    
Article Type: Original Research | Subject: Pharmaceutical Biotechnology
Received: 2018/12/18 | Accepted: 2019/02/2 | Published: 2019/12/21

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