Volume 11, Issue 2 (2020)                   JMBS 2020, 11(2): 127-136 | Back to browse issues page

‎ 20.1001.1.23222115.1399.11.2.3.4

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etemadi N, Akhavan sepahi A, Yazdian F, mohebali G. The Optimization of Culture Medium Bacillus thermoamylovorance Strain EAMYO by RSM and Improvement Biodesulfurization activity by Starch/Iron Nano Particles. JMBS 2020; 11 (2) :127-136
URL: http://biot.modares.ac.ir/article-22-30612-en.html
The Optimization of Culture Medium Bacillus thermoamylovorance Strain EAMYO by RSM and Improvement Biodesulfurization activity by Starch/Iron Nano Particles
Narges Etemadi1 , Abbas Akhavan sepahi1 , Fatemeh Yazdian * 2, Ghasemali Mohebali3
1- Microbiology Group, Faculty of Life Science, North Tehran Branch, Islamic Azad University, Tehran, Iran.
2- Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran , yazdian@ut.ac.ir
3- Microbiology and Biotechnology Research Group, Research Institute of Petroleum Industry, Tehran, Iran
Abstract:   (3647 Views)
The combustion of fossil fuels containing sulfur results in the release of sulfur dioxide into the atmosphere and environmental pollution. Hence, the researchers focused on the biological desulfurization method. Dibenzothiophene is used as the model molecule to study the ability of the desulfurization of microorganisms. The most suitable sources of carbon, nitrogen and sulfur concentration optimized by response surface method to obtain the highest cell growth and biological desulfurization activity. The performance of iron nanoparticles on the growth and biodesulfurization activity of thermophilic bacterium Bacillus thermoamylovorans strain EAMYO was investigated. Characterization of starch-modified iron nanoparticles was performed by TEM, SEM. The images of TEM and SEM of starch / Iron nanoparticles showed that the Fe3O4 and Fe0 nanoparticles were 20 and 30 nm, respectively. The investigating the growth of microorganism in the presence of iron nanoparticles showed that these nanoparticles not only did not have a toxic effect on microorganism growth, but also increased the growth of microorganism in 96 h (OD 660 = 1.864, 1.896 respectively in the presence of nanoparticles Fe0 and Fe3O4), while the highest rate of growth in the absence of nanoparticles in 96 h (OD660 = 1.51). Also, the activity of desulfurization in the presence of starch/Fe0 nanoparticles and starch/Fe3O4 / starch increased by 26.52% and 10.75%, respectively, compared to the cells without the coating of iron nanoparticles.
 
Keywords: Thermophilic bacterium, Bio-desulfurization, RSM, Starch modified iron nanoparticles.
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Article Type: Original Research | Subject: Industrial Biotechnology
Received: 2019/02/20 | Accepted: 2019/12/11 | Published: 2020/06/6
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