Volume 11, Issue 4 (2020)                   JMBS 2020, 11(4): 43-54 | Back to browse issues page

‎ 20.1001.1.23222115.1399.11.4.9.4

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Golestanipour A, Aalami A, Nikkhah M, Hosseinkhani S. Gene delivery to tobacco cells using single walled carbon nanotubes: a new generation of gene transfer vehicles. JMBS 2020; 11 (4) :43-54
URL: http://biot.modares.ac.ir/article-22-49529-en.html
Gene delivery to tobacco cells using single walled carbon nanotubes: a new generation of gene transfer vehicles
Arezoo Golestanipour1 , Ali Aalami * 2, Maryam Nikkhah3 , Saman Hosseinkhani4
1- Department of Plant Biotechnology, University Campus 2, University of Guilan, Rasht, Iran
2- Department of Biotechnology, Faculty of Agricultural Sciences, University of Guilan, , ali_aalami@guilan.ac.ir
3- Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
4- Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:   (1646 Views)
Developing a technique for efficient and safe gene delivery to plant cells is a fundamental aim of plant biotechnology. Agrobacterium mediated transformation as the most common and practical method in plant gene delivery has considerable difficulties such as limitation in applicable for plant species. In recent years several new methods have been suggested, although none of them could be a good replacement. The use of nanotechnology has been provided new solutions to overcome the limitations of biotechnology. Designing biocompatible nanostructures for passing cell barriers and targeted delivery of cargo has improved the biological achievements. In this research the capability of arginine functionalized single-walled carbon nanotube (Arg-SWNT) as a new carrier to transfer plasmid DNA, which codes green fluorescent protein (GFP) to tobacco suspension cells, has been investigation. It is suggested that single-walled carbon nanotubes can pass through cell wall pores and plasma membrane while it carries plasmid DNA along with. The fluorescence microscopy images illustrate the success of gene delivery by Arg-SWNT
Keywords: Arginine, Green fluorescent protein, Single-walled carbon nanotube
Full-Text [PDF 534 kb]   (1035 Downloads)    
Article Type: Original Research | Subject: Nanotechnology
Received: 2021/01/25 | Accepted: 2020/11/30 | Published: 2020/11/30
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