Volume 14, Issue 3 (2024)                   JMBS 2024, 14(3): 0-0 | Back to browse issues page

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Fasihi A, Nemati H, Kabiri F, Hasheminasab H, Mohamad Soltani B. Hsa-miR-424 by targeting APC increases Wnt signaling activates in colorectal cancer. JMBS 2024; 14 (3)
URL: http://biot.modares.ac.ir/article-22-61129-en.html
1- Molecular Genetics Department, Faculty of Biological Sciences, Tarbiat Modares University
2- Genetics Department Faculty of Biological Sciences , soltanib@modares.ac.ir
Abstract:   (254 Views)
The activity of Wnt signaling pathway is increased in colorectal cancer. For this reason, finding new positive and negative regulators for this pathway is a treatment and diagnostic strategy of colorectal cancer. Our bioinformatics analysis indicated that hsa-miR-424 (miR-424) could be a possible regulator of the Wnt signaling pathway. Accordingly, the expression level of miR-424 in colorectal cancer tissues was elevated compared with normal pairs and the results of RT-qPCR showed a significant increase in miR-424 expression (p < 0.01). Then, molecular analyzes using Top/Fop Flash and RT-qPCR techniques indicated that miR-424 overexpression leads to increased Wnt pathway activity in the SW480 cell line. In addition, the small molecules IWP-2 and PNU-74654 were used to inhibit the Wnt signaling pathway, and the miR-424 overexpression suggested that exert its effect on the level of β-catenin complex degradation. Then, dual-luciferase assay validated the interaction between miR-424 and APC. Overall, our results suggest miR-424 is a positive regulator of the Wnt signaling pathway, and it could be a possible prognosis for colorectal cancer.
 
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Article Type: Original Research | Subject: Molecular biotechnology
Received: 2022/04/26 | Accepted: 2022/11/15 | Published: 2024/05/29

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