Volume 2, Issue 1 (2011)                   JMBS 2011, 2(1): 0-0 | Back to browse issues page

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Darvishi F, Nahvi I, Zarkesh H. Investigation of nitrogen sources on lipase production of Yarrowia lipolytica yeast. JMBS 2011; 2 (1)
URL: http://biot.modares.ac.ir/article-22-82-en.html
Investigation of nitrogen sources on lipase production of Yarrowia lipolytica yeast
Farshad Darvishi * 1, Iraj Nahvi2 , Hamid Zarkesh2
1- Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh 55181-83111, Maragheh
2- Isfahan
Abstract:   (13210 Views)
Lipase is used in the production of detergents, cosmetics, pharmaceuticals, flavour enhancers and foods. The lipase of yeast Yarrowia lipolytica can be used for production of important class of chemical intermediates in the pharmaceutical industry. Lipase production depends on media composition and environmental conditions. Y. lipolytica DSM 3286 strain was cultured on media containing different organic and inorganic nitrogen sources. Lipase production was investigated by measuring biomass and lipase activity was detected by ρ-nitrophenyl laurate (PNPL) spectrophotometric assay method at various times within a period of 7 days. In this study, the effect of different nitrogen sources was investigated on Y. lipolytica DSM 3286 lipase production. The maximal lipase production (34.7 U/ml after 48 h) was detected in medium containing yeast extract as nitrogen source. The optimum temperature and pH of the enzyme activity were 37 °C and 7, respectively. The final goal of this study is to develop and optimize lipase production by Y. lipolytica for use in pharmaceutical industry.
Keywords: Yarrowia lipolytica, lipase, Nitrogen sources, optimization
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Article Type: Original Manuscript | Subject: biochemistry|Biotechnology|Food Science and Technology
Received: 2010/11/5 | Accepted: 2011/02/23 | Published: 2011/09/19
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