Investigating the association between HLA-DQB1*03 with multiple sclerosis (MS) in Tehran

Salehi Siavashani, Marziyeh; Nakhai Sistani, Ruhollah; Panahi, Alireza

Investigating the association between HLA-DQB1*03 with multiple sclerosis (MS) in Tehran

Document Type : Brief Communication

Authors

Marziyeh Salehi Siavashani ¹

Ruhollah Nakhai Sistani ¹

alireza panahi ²

¹ University of kashan

² University of Mohaghegh Ardabili

Abstract

Aim: Multiple sclerosis is important in Iran because of its high prevalence and low age of onset. It exerts a large burden on affected people and the health care system. Studies have shown that the genetic content of humans has a critical role in MS. The histocompatibility loci which their products present the foreign antigenic peptides for detection by lymphocytes are of MS associated genetic elements. In this study, the association of HLA-DQB1*03 with MS was studied in Tehran, using a PCR-based system.

Methods: In this case-control study, a total of 367 blood samples were collected from 172 patients and 195 healthy people. Both groups were similar in age and gender. Blood DNA was extracted, and PCR technique was used to identify the presence of the allele.

Results: In this study, the HLA-DQB1*03 allele in men was significantly higher than that of women (p = 0.002). Also, the allele was less frequent in patients than in healthy subjects (53% versus 67%), and this difference is significant (p = 0.02).

Conclusions: The DQB1*03 allele is significantly lower in patients with MS than in healthy people, and this relationship is more pronounced in male subjects. Therefore, it seems that this allele plays a protective role against MS disease.

Keywords

Multiple sclerosis

Gene Frequency

Polymorphism

Polymerase Chain Reaction

20.1001.1.23222115.1401.13.2.4.9

Subjects

Molecular biotechnology

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